Medical College of Nankai University, Tianjin, China.
Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China.
Front Cell Infect Microbiol. 2022 Aug 25;12:983169. doi: 10.3389/fcimb.2022.983169. eCollection 2022.
Patients with inflammatory bowel disease (IBD) are often accompanied with some cognitive impairment, but the mechanism is unclear. By orally exposing honeybees () to IBD-associated LF82 (LF82), and non-pathogenic MG1655 (MG1655) as the normal strain, we investigated whether and how LF82 induces enteritis-like manifestations and cognitive behavioral modifications in honeybees using multiparametric analysis. LF82 significantly increased gut permeability, impaired learning and memory ability in olfactory proboscis extension response conditioning, and shortened the lifespan of honeybees. Compared to MG1655, LF82 reduced the levels of tryptophan metabolism pathway substances in the honeybee gut. LF82 also upregulated genes involved in immune and apoptosis-related pathways and downregulated genes involved in G protein-coupled receptors in the honeybee brain. In conclusion, LF82 can induce enteritis-like manifestations and cognition impairment through gut metabolites and brain transcriptome alteration in honeybees. Honeybees can serve as a novel potential model to study the microbiota-gut-brain interaction in IBD condition.
患有炎症性肠病 (IBD) 的患者常伴有一定程度的认知障碍，但具体机制尚不清楚。本研究通过口服使蜜蜂接触 IBD 相关的 LF82（LF82）和非致病性 MG1655（MG1655）（正常株），采用多参数分析，研究 LF82 是否以及如何在蜜蜂中引起类似肠炎的表现和认知行为改变。结果表明 LF82 显著增加了肠道通透性，损害了嗅觉触须延伸反应条件下的学习和记忆能力，并缩短了蜜蜂的寿命。与 MG1655 相比，LF82 降低了蜜蜂肠道中色氨酸代谢途径物质的水平。LF82 还上调了蜜蜂脑中与免疫和细胞凋亡相关途径相关的基因，下调了与 G 蛋白偶联受体相关的基因。综上所述，LF82 可通过改变蜜蜂肠道代谢物和脑转录组引起类似肠炎的表现和认知障碍。蜜蜂可以作为研究 IBD 条件下微生物群-肠道-大脑相互作用的新型潜在模型。
