[精氨酸加压素对离体大鼠脂肪细胞释放游离脂肪酸的刺激作用]

[Stimulating effect of arginine vasopressin on the release of free fatty acids by isolated rat adipose cells].

作者信息

Abel'son Iu O

出版信息

Fiziol Zh SSSR Im I M Sechenova. 1987 Apr;73(4):537-40.

Abstract

Arginine vasopressin (AVP) action on the lipolysis, both the basic and induced with noradrenaline, was studied in adipocytes isolated from epididymal fat pads of Wistar rats sacrificed after 24-hr food deprivation. Adipocytes were exposed to hormones for 10 or 15 min. Lipolysis was estimated by measuring FFA release. AVP alone in relatively small concentrations (8 X 10(-9) M and 8 X 10(-11) M) stimulated FFA release by 39.6% and 56.3%, resp. Higher concentrations (8 X 10(-7) M) exerted no effect. Having been added to noradrenaline, however, AVP in concentrations 8 X 10(-7) M and 8 X 10(-9) M decreased lipolytic effect of the former, although the decrease was statistically insignificant. Whether the activating effect of AVP is due to the stimulation of lipolysis or to changes in membrane permeability for FFA, is to be elucidated.

摘要

研究了精氨酸加压素（AVP）对基础脂解以及去甲肾上腺素诱导的脂解的作用，实验使用的脂肪细胞取自禁食24小时后处死的Wistar大鼠附睾脂肪垫。脂肪细胞与激素接触10或15分钟。通过测量游离脂肪酸（FFA）释放来评估脂解。单独使用相对低浓度（8×10⁻⁹ M和8×10⁻¹¹ M）的AVP分别使FFA释放增加39.6%和56.3%。较高浓度（8×10⁻⁷ M）则无作用。然而，添加到去甲肾上腺素中时，浓度为8×10⁻⁷ M和8×10⁻⁹ M的AVP会降低去甲肾上腺素的脂解作用，尽管这种降低在统计学上不显著。AVP的激活作用是由于刺激脂解还是由于FFA膜通透性的改变，尚待阐明。