Romppanen U, Rantala I, Lauslahti K, Reunala T
Dermatologica. 1987;175(1):33-40. doi: 10.1159/000248779.
Fifteen women with lichen sclerosus (LS) of the vulva were treated for 3 months with 0.6 mg/kg of etretinate. The clinical response was good in 11/15 of the patients and, in agreement with this, light microscopy showed decreasing atrophy and hyperkeratosis together with subsiding inflammatory changes. However, orcein staining demonstrated that even after etretinate treatment, elastic fibers were still absent in the upper dermis with only few fibers being detectable in some specimens. Conventional electron microscopy and immunoelectron microscopy were used to examine these changes more in detail. Antiserum to the microfibrillar part of the elastic fibers confirmed that only remnants of microfibrillar coat persisted in the upper dermis and that deeper in the dermis, elastic fibers were porous and fragmented. These findings demonstrate that despite the normalization of clinical and certain histological parameters during etretinate treatment, the initial elastic fiber damage persists both at the light- and electronmicroscopic levels.
15名患有外阴硬化性苔藓（LS）的女性接受了3个月的维甲酸治疗，剂量为0.6mg/kg。15名患者中有11名临床反应良好，与此一致的是，光镜检查显示萎缩和角化过度减轻，炎症变化消退。然而，地衣红染色显示，即使在维甲酸治疗后，真皮上层仍无弹性纤维，仅在一些标本中可检测到少量纤维。使用传统电子显微镜和免疫电子显微镜更详细地检查这些变化。针对弹性纤维微纤维部分的抗血清证实，仅微纤维包膜的残余物存在于真皮上层，而在真皮深层，弹性纤维多孔且破碎。这些发现表明，尽管在维甲酸治疗期间临床和某些组织学参数恢复正常，但最初的弹性纤维损伤在光镜和电镜水平上仍然存在。
