Key Laboratory of Targeting Therapy and Diagnosis for Critical Diseases of Henan Province, School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, Henan, China.
China National Tobacco Quality Supervision and Test Center, Zhengzhou, Henan, China.
J Chromatogr A. 2022 Oct 11;1681:463480. doi: 10.1016/j.chroma.2022.463480. Epub 2022 Sep 6.
In this study, a novel kapok fiber-supported liquid extraction (KF-SLE) method was developed for conveniently extracting analytes from oil samples. Natural kapok fiber without any pretreatment was directly used as an oil support medium. The extraction device was conveniently constructed by directly packing some kapok fibers into a syringe tube. Due to the fibrous property of the kapok fiber, no filter plate was needed. The cost of a KF-SLE device was as low as 0.5 CNY. The KF-SLE process was conveniently conducted using a simple three-step protocol: (1) the oil sample without any pretreatment including dilution was added directedly; (2) then, the oil-immiscible extractant was added; (3) after waiting a certain time for static extraction, the extractant was eluted out by pressing the kapok fibers with the syringe plunger. The extractant could be directly transferred for subsequent instrumental detection. For the feasibility and proof-of-concept study, the method was applied to quantify four synthetic flavor chemicals in edible oils. Satisfied quantification results were obtained with the correlation coefficient (R) being greater than 0.996, the relative recoveries ranging from 92.90% to 107.53% and intra- and inter-day RSDs being less than 7.56%. All in all, for the first time, the SLE technique was expanded to process oil samples and the method has the characteristics of low cost, environmental friendliness, high sample processing throughput and ease of automation, offering a promising approach for edible oil sample preparations.
在这项研究中，开发了一种新型的木棉纤维支撑液萃取（KF-SLE）方法，用于方便地从油样中提取分析物。未经任何预处理的天然木棉纤维直接用作油支撑介质。通过直接将一些木棉纤维装入注射器管中，方便地构建了萃取装置。由于木棉纤维的纤维性质，不需要过滤板。KF-SLE 装置的成本低至 0.5 元人民币。KF-SLE 过程通过简单的三步方案方便地进行：（1）直接添加未经任何预处理的油样，包括稀释；（2）然后，加入与油不混溶的萃取剂；（3）等待一定时间进行静态萃取后，用注射器柱塞将木棉纤维压出，洗脱萃取剂。萃取剂可直接用于后续的仪器检测。为了可行性和概念验证研究，该方法应用于定量食用油脂中的四种合成香料化学品。得到了令人满意的定量结果，相关系数（R）大于 0.996，相对回收率在 92.90%至 107.53%之间，日内和日间 RSD 小于 7.56%。总之，首次将 SLE 技术扩展到处理油样，该方法具有成本低、环保、高样品处理通量和易于自动化的特点，为食用油样品制备提供了一种有前途的方法。
