Shiraz Nephro-Urology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Shiraz Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Virol J. 2022 Sep 12;19(1):147. doi: 10.1186/s12985-022-01880-5.
Human cytomegalovirus (CMV) can establish a latent infection with periodic or sporadic reactivation after the first infection happens. Primary and recurrent infection, results in different problems in patients with impaired or immature immune systems, such as kidney transplant recipients (KTRs). MicroRNAs (miRNAs, miRs) are important regulatory molecules in the outcome of CMV-infected KTRs. Therefore, in this study the expression level of CMV miRNAs were evaluated in active vs. latent CMV infected KTRs.
Expression of viral miRNAs were studied in 61 KTRs which were divided into 30 active CMV and 31 latent CMV infected individuals. In order to study the expression level of selected miRNAs, SYBR Green Real-time PCR technique was exploited. Also, mature miRNAs expression level that were produced from one precursor, studied both in active and latent situations.
Among studied miRNAs' expression level, CMV miR-UL112-3p/5p, -UL22A-3p/5p, -US25-1-5p, -US25-2-3p/5p, -UL36-3p/5p and -UL70-3p showed significant increase in active CMV infected KTRs in comparison to latent ones. The ROC curve analysis results for miR-UL112-3p, -UL22A-3p, -US25-2-3p, -UL36-3p and -UL70-3p showed significant difference between two studied patient groups.
This study revealed an extremely high expression level in CMV miR-UL112-3p/5p, -UL22A-3p/5p, -US25-1-5p, -US25-2-3p/5p, -UL36-3p/5p and -UL70-3p in active CMV infected KTRs in comparison to latent ones. Further studies might help in finding the capability of miRNAs to differentiate active from latent stage of CMV infection in KTRs.
人类巨细胞病毒（CMV）在初次感染后可建立潜伏感染，并周期性或偶发性再激活。原发和复发感染会导致免疫系统受损或不成熟的患者出现不同问题，如肾移植受者（KTR）。微小 RNA（miRNA，miRs）是 CMV 感染 KTR 结局的重要调节分子。因此，本研究评估了活跃和潜伏 CMV 感染 KTR 中 CMV miRNA 的表达水平。
对 61 名 KTR 进行病毒 miRNA 表达研究，分为 30 名活跃 CMV 感染和 31 名潜伏 CMV 感染个体。为了研究选定 miRNA 的表达水平，采用 SYBR Green 实时 PCR 技术。此外，还研究了在活跃和潜伏状态下由一个前体产生的成熟 miRNA 的表达水平。
在所研究的 miRNA 表达水平中，CMV miR-UL112-3p/5p、-UL22A-3p/5p、-US25-1-5p、-US25-2-3p/5p、-UL36-3p/5p 和 -UL70-3p 在活跃 CMV 感染 KTR 中表达显著增加与潜伏者相比。miR-UL112-3p、-UL22A-3p、-US25-2-3p、-UL36-3p 和 -UL70-3p 的 ROC 曲线分析结果显示，两组研究患者之间存在显著差异。
本研究表明，与潜伏 CMV 感染 KTR 相比，活跃 CMV 感染 KTR 中 CMV miR-UL112-3p/5p、-UL22A-3p/5p、-US25-1-5p、-US25-2-3p/5p、-UL36-3p/5p 和 -UL70-3p 的表达水平极高。进一步的研究可能有助于发现 miRNA 区分 KTR 中 CMV 感染活跃期和潜伏期的能力。
