Nduka E, Todd H, Hertelendy F
Gen Comp Endocrinol. 1987 Jun;66(3):369-73. doi: 10.1016/0016-6480(87)90246-2.
Addition of serum (0.02-2%, v/v) from laying hens to dispersed granulosa cells isolated from the largest and second-largest ovarian follicles of regularly laying hens significantly potentiated both ovine- and chicken LH-induced progesterone production. A similar treatment with mammalian sera, on the other hand, tended to suppress steroidogenesis, though not to a statistically significant degree. When steroidogenesis was stimulated by forskolin or 8-bromo-cyclic AMP, both of which act at postreceptor sites, the presence of either avian or mammalian sera enhanced steroidogenesis over controls stimulated by agonist alone. It is suggested that serum lipoproteins potentiate steroidogenesis, but that factors present in mammalian sera interfere with the action of LH at a step preceding cyclic AMP generation.
向从正常产蛋母鸡最大和第二大卵巢卵泡中分离出的分散颗粒细胞中添加（0.02 - 2%，v/v）产蛋母鸡血清，能显著增强绵羊和鸡促黄体生成素诱导的孕酮生成。另一方面，用哺乳动物血清进行类似处理则倾向于抑制类固醇生成，尽管在统计学上没有显著差异。当用福斯可林或8 - 溴环磷酸腺苷刺激类固醇生成时（二者均作用于受体后位点），禽类或哺乳动物血清的存在均能增强类固醇生成，超过单独用激动剂刺激的对照组。提示血清脂蛋白能增强类固醇生成，但哺乳动物血清中存在的因子在环磷酸腺苷生成之前的步骤干扰促黄体生成素的作用。
