Pak J Biol Sci. 2022 Jan;25(8):741-747. doi: 10.3923/pjbs.2022.741.747.
<b>Background and Objective:</b> The growth hormone (GH) gene plays a role in meat growth and has been shown to increase the growth rate and carcass composition after being given GH. For this function, this gene is used as a strong candidate for genetic markers for meat growth traits. The research objective was to map the growth hormone (GH) gene of the bodyweight of Krui cattle in the Pesisir Barat Regency. <b>Materials and Methods:</b> This research used 30 blood samples of 30 Krui cattle. The method used was by taking quantitative data and blood samples from adult Krui cattle in Pesisir Barat Regency and then the blood samples were analyzed by DNA isolation method. PCR amplification used was a pair of GH-Forward primers: 5 'ATC CAC ACC CCC TCC ACA CAGT 3' and GH- reverse: 5 'CAT TTT CCA CCC TCC CCT ACA G 3', as well as digestion using the RFLP method at the Laboratory of Animal Breeding and Genetics of Universitas Gadjah Mada, Yogyakarta. Association between genotype and body weight was analyzed descriptively. <b>Results:</b> The results showed that Krui cattle had polymorphic genes with three genotypes found, namely: CC, CT and TT. Cattle with CT genotype had the largest average body weight or meat production compared to those with other genotypes. <b>Conclusion:</b> These results indicated that the GH gene identifier has strong evidence that it can be used as a selection tool with the help of genotypes of body weight traits of Krui meat production in the Pesisir Barat Regency. Krui cattle with CT genotype can be developed further because it has high economic value with high average body weight and meat production.
生长激素（GH）基因在肉质生长中起作用，并且在给予 GH 后已显示出可提高生长速度和胴体组成。由于此功能，该基因被用作肉质生长性状的遗传标记的有力候选者。本研究的目的是对西加里曼丹县克鲁伊牛的生长激素（GH）基因进行定位。材料与方法：本研究使用了 30 份来自西加里曼丹县 30 头克鲁伊牛的血液样本。所使用的方法是通过从西加里曼丹县成年克鲁伊牛中获取定量数据和血液样本，然后通过 DNA 分离方法分析血液样本。使用的 PCR 扩增是一对 GH-正向引物：5'ATC CAC ACC CCC TCC ACA CAGT 3'和 GH-反向引物：5'CAT TTT CCA CCC TCC CCT ACA G 3'，以及在日惹加查马达大学动物繁殖与遗传实验室使用 RFLP 方法进行消化。使用描述性方法分析基因型与体重之间的关系。结果：结果表明，克鲁伊牛具有三种基因型的多态性基因，分别为：CC、CT 和 TT。与其他基因型相比，具有 CT 基因型的牛的平均体重或肉质产量最大。结论：这些结果表明，GH 基因鉴定具有很强的证据表明，它可以作为一种选择工具，帮助选择西加里曼丹县克鲁伊牛的体重性状，以提高其肉质产量。具有 CT 基因型的克鲁伊牛可以进一步发展，因为它具有高平均体重和肉质产量的高经济价值。
