Amako K, Matsuguchi M, Takeya K, Tatsuta E, Takagi Y
Antimicrob Agents Chemother. 1978 Sep;14(3):488-92. doi: 10.1128/AAC.14.3.488.
A bacteriocin produced by strain 100052 of Shigella sonnei (shigellacin 52) was purified about 80-fold from a mitomycin C-induced culture supernatant. The purified preparation gave a single band on sodium dodecyl sulfate-gel electrophoresis with an approximate molecular weight of 10,000. The negatively stained electron micrograph of the purified bacteriocin preparation revealed the existence of three different particles. They were a small cylindrical tube (4.2 by 6.0 nm), a ring-shaped particle and a filamentous structure. The molecular weight calculated from the shape of the small tube was approximately 50,000. The latter two particles appeared to be constructed from subunits which were morphologically similar to the cylindrical tube. These results suggested that the morphological subunit of shigellacin 52 consisted of five chemical subunits with molecular weights of 10,000 each, and it assembled into two types of polymers, a ring-shaped particle and a filamentous structure, which seemed to be the main components of the purified shigellacin 52 preparation.
从经丝裂霉素C诱导的培养上清液中，将宋内志贺氏菌100052菌株产生的一种细菌素（志贺菌素52）纯化了约80倍。纯化后的制剂在十二烷基硫酸钠凝胶电泳上呈现单一条带，其近似分子量为10,000。纯化的细菌素制剂的负染电子显微镜照片显示存在三种不同的颗粒。它们是一个小圆柱形管（4.2×6.0纳米）、一个环形颗粒和一个丝状结构。根据小管所测得的分子量约为50,000。后两种颗粒似乎由形态上与圆柱形管相似的亚基构成。这些结果表明，志贺菌素52的形态学亚基由五个分子量各为10,000的化学亚基组成，并且它组装成两种类型的聚合物，即环形颗粒和丝状结构，这似乎是纯化的志贺菌素52制剂的主要成分。
