Cas9 古老核酶的快照。
Snapshots of a tiny ancestral nuclease of Cas9.
机构信息
Department of Biological Chemistry, University of Michigan, Ann Arbor, MI 48109, USA.
Department of Biological Chemistry, University of Michigan, Ann Arbor, MI 48109, USA.
出版信息
Trends Biochem Sci. 2023 Jan;48(1):9-10. doi: 10.1016/j.tibs.2022.08.008. Epub 2022 Sep 10.
PMID:36100522
Abstract
High-resolution structures solved by Schuler et al. shed light on how Cas9's evolutionary ancestor IscB operates as an RNA-guided nuclease. With only two-fifths the size of Cas9, IscB holds great promise for alleviating the cargo size constraint of in vivo CRISPR delivery.
摘要
舒勒等人解析的高分辨率结构阐明了 Cas9 的进化前体 IscB 如何作为一种 RNA 引导的核酸酶发挥作用。IscB 的大小只有 Cas9 的五分之二，它为缓解体内 CRISPR 递送的载体大小限制带来了很大的希望。
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