Institute of Food Safety, Chinese Academy of Inspection & Quarantine, Beijing 100176, China.
School of Food Science and Engineering, Qingdao Agricultural University, Qingdao, Shandong 266109, China.
J Agric Food Chem. 2022 Sep 28;70(38):12211-12219. doi: 10.1021/acs.jafc.2c02868. Epub 2022 Sep 13.
Zearalenone has attracted worldwide attention due to its toxic properties and threat to public health. A rapid determination method for zearalenone and its derivatives by hydrophilic covalent organic frameworks coated steel sheet (HCOFCS) combined with ambient mass spectrometry (AMS) was developed. The HCOFCS behaved as both a tip for solid-phase microextraction and a solid substrate for electrospray ionization mass spectrometry (ESI-MS). To evaluate the HCOFCS-ESI-MS method, five zearalenone and its derivatives in milk samples were determined, including zearalenone (ZEA), α-zearalenol (α-ZEL), β-zearalenol (β-ZEL), α-zearalanol (α-ZAL), and β-zearalanol (β-ZAL). After the extraction procedure, the HCOFCS was directly added with a high voltage for ESI-MS, and the analysis could be completed within 1 min. The developed method showed good linearity in the range 0.1-100 μg/L with a coefficient of determination () > 0.9991. The limits of detection (LODs) and limits of quantitation (LOQs) ranged from 0.05 to 0.1 and 0.2 to 0.3 μg/L, respectively. The results demonstrated that the HCOFCS combined with ESI-MS can be used for the rapid and sensitive determination of trace ZEA and its derivatives in milk samples with satisfactory recoveries from 80.58% to 109.98% and reproducibility with relative standard deviations (RSDs) no more than 11.18%. Furthermore, HCOFCS showed good reusability, which could reuse at least 10 extraction cycles with satisfactory adsorption performance.
玉米赤霉烯酮因其毒性和对公众健康的威胁而引起了全世界的关注。本研究开发了一种基于亲水共价有机框架涂覆钢板（HCOFCS）结合大气压力质谱（AMS）快速测定玉米赤霉烯酮及其衍生物的方法。HCOFCS 既可用作固相微萃取的尖端，也可用作电喷雾电离质谱（ESI-MS）的固体基质。为了评估 HCOFCS-ESI-MS 方法，本研究在牛奶样品中测定了五种玉米赤霉烯酮及其衍生物，包括玉米赤霉烯酮（ZEA）、α-玉米赤霉烯醇（α-ZEL）、β-玉米赤霉烯醇（β-ZEL）、α-玉米赤醇（α-ZAL）和β-玉米赤醇（β-ZAL）。在提取过程后，将 HCOFCS 直接施加高压进行 ESI-MS，分析可在 1 分钟内完成。该方法在 0.1-100 μg/L 范围内具有良好的线性关系，相关系数（）大于 0.9991。检出限（LOD）和定量限（LOQ）范围分别为 0.05-0.1 和 0.2-0.3 μg/L。结果表明，HCOFCS 结合 ESI-MS 可用于快速灵敏地测定牛奶样品中痕量玉米赤霉烯酮及其衍生物，回收率为 80.58%-109.98%，相对标准偏差（RSD）不超过 11.18%，重现性良好。此外，HCOFCS 具有良好的可重复使用性，至少可重复使用 10 次，且具有令人满意的吸附性能。
