使用高效价抗血清和灵敏的脲酶酶联免疫吸附测定法测定鸟类和哺乳动物血清学性别特异性（Sxs）抗原（“H-Y抗原”）
Determination of the serological sex-specific (Sxs) antigen ("H-Y antigen") in birds and mammals using high-titer antisera and a sensitive urease ELISA.
作者信息
Bradley M P, Ebensperger C, Wiberg U H
出版信息
Hum Genet. 1987 Aug;76(4):352-6. doi: 10.1007/BF00272444.
A rapid, sensitive, and reproducible enzyme-linked immunosorbent assay (ELISA), for the detection of the serological sex-specific (Sxs) antigen (formerly termed H-Y antigen; see Introduction), is described. This assay uses bovine testes extract as the solid phase antigen, and high-titer anti-Sxs antisera and a urease-conjugated anti rat-IgG as the first and second antibody respectively. The urea containing substrate causes a pH shift in a positive reaction, which in turn is visualized by the use of bromocresol purple as a pH indicator. The method, and some representative applications of it, are described in detail.
本文描述了一种快速、灵敏且可重复的酶联免疫吸附测定（ELISA）方法，用于检测血清学性别特异性（Sxs）抗原（以前称为H-Y抗原；见引言）。该测定法使用牛睾丸提取物作为固相抗原，分别使用高效价抗Sxs抗血清和脲酶偶联的抗大鼠IgG作为第一抗体和第二抗体。含尿素的底物在阳性反应中会引起pH值变化，进而通过使用溴甲酚紫作为pH指示剂来显现。详细描述了该方法及其一些代表性应用。
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