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组织样本薄层的定性调查分析。人体肺组织中的重金属痕迹。

Qualitative survey analysis of thin layers of tissue samples. Heavy metal traces in human lung tissue.

作者信息

von Bohlen A, Klockenkämper R, Otto H, Tölg G, Wiecken B

出版信息

Int Arch Occup Environ Health. 1987;59(4):403-11. doi: 10.1007/BF00405284.

DOI:10.1007/BF00405284
PMID:3610340
Abstract

A special X-ray fluorescence spectrometric method has been used for the analyses of thin layers of tissue samples. This method [called Total-Reflection X-ray Fluorescence (TXRF)] gives qualitative information about numerous trace elements of physiological and environmental interest. Microtome cuts of frozen lung tissue samples are placed on quartz glass targets and excited to X-ray fluorescence by the primary beam of a molybdenum tube. This method is of interest due to the very short time needed for sampling, sample preparation and simultaneous measurement. It could be a helpful procedure in pathological and occupational medicine if a decision has to be made as to whether or not a tissue sample is extremely dust loaden.

摘要

一种特殊的X射线荧光光谱法已被用于分析组织样本的薄层。这种方法[称为全反射X射线荧光(TXRF)]可提供有关许多具有生理和环境意义的微量元素的定性信息。将冷冻肺组织样本的切片放在石英玻璃靶上,并用钼管的初级光束激发产生X射线荧光。由于采样、样品制备和同时测量所需的时间非常短,所以这种方法很有意义。如果必须决定一个组织样本是否含有极高的粉尘负荷,那么它在病理和职业医学中可能是一个有用的程序。

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本文引用的文献

1
Optical flats for use in x-ray spectrochemical microanalysis.用于X射线光谱化学微分析的光学平面。
Rev Sci Instrum. 1971 Jul;42(7):169-70. doi: 10.1063/1.1685282.
2
Trace analysis to determine heavy metal load in lung tissue. A contribution to substantiation of occupational hazards.
Int Arch Occup Environ Health. 1986;58(1):27-34. doi: 10.1007/BF00378537.