利用荧光蛋白进行 DNA 可视化。

DNA Visualization Using Fluorescent Proteins.

机构信息

College of Agriculture, Yanbian University, Yanji, Jilin Province, China.

Department of Chemistry and Program of Integrated Biotechnology, Sogang University, Seoul, Korea.

出版信息

Methods Mol Biol. 2023;2564:223-246. doi: 10.1007/978-1-0716-2667-2_11.

DOI:10.1007/978-1-0716-2667-2_11

PMID:36107345

Abstract

DNA binding fluorescent proteins are a powerful tool for single-molecule visualization. In this chapter, we discuss a protocol for the synthesis of DNA binding fluorescent proteins and visualization of single DNA molecules. This chapter includes stepwise methods for molecular cloning, reversible staining, two-color staining, sequence-specific staining, and microscopic visualization of single DNA molecules in a microfluidic device. This content will be useful for DNA characterization using DNA binding fluorescent proteins and its visualization at the single-molecule level.

摘要

DNA 结合荧光蛋白是单分子可视化的有力工具。在本章中，我们讨论了合成 DNA 结合荧光蛋白和可视化单 DNA 分子的方案。本章包括分子克隆、可逆染色、双色染色、序列特异性染色和在微流控装置中单 DNA 分子的微观可视化的分步方法。这部分内容将有助于使用 DNA 结合荧光蛋白进行 DNA 特征分析及其在单分子水平的可视化。