Center for Space Medicine and Extreme Environments Berlin, Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, Institute of Physiology, Berlin, Germany.
Laboratory of Translational Research "Stress and Immunity", Department of Anesthesiology, Ludwig-Maximilians-University, Munich, Germany.
J Appl Physiol (1985). 2022 Nov 1;133(5):1119-1135. doi: 10.1152/japplphysiol.00180.2022. Epub 2022 Sep 15.
The endothelial glycocalyx maintains vascular structure and may be subject to shedding during inflammation and also during high-intensive exercise. There are no studies on shedding during ultra-endurance exercise. The "Yukon Arctic Ultra" (YAU) is one of the longest and coldest ultramarathons and its impact on glycocalyx shedding was investigated. Thirteen adults (38.92 ± 8.67 yr, 6 females) of YAU editions 2015-2019 completed 657.03 ± 71.65 km at a moving velocity of 4.17 ± 0.62 km/h. Mean daily temperatures ranged from -12.6°C to -30.5°C. Glycocalyx elements heparan sulfate, hyaluronan, and syndecan CD-138 were quantified from serum at start, 277 km, 383 km, and 690 km. Cortisol, C-reactive protein, creatine kinase, and N-terminal-prohormone of brain natriuretic peptide were also quantified. Seven YAU volunteers (36.14 ± 11.04 yr, 5 females) served as control. There were no time-changes among the control. Among finishers, there was a significant increase for hyaluronan and a significant decrease for syndecan CD-138. Values were greater among female finishers for heparan sulfate at start, 383 km, and 690 km, and among male finishers for hyaluronan at 277 km. Values for syndecan CD-138 were greater among older finishers at all timepoints. There were weak significant correlations ( < 0.215) between hyaluronan and distance, creatine kinase, and NT-Pro BNP, respectively. Shedding of glycocalyx elements is shown among participants of the YAU. Greater shedding of heparan sulfate among female, greater increases of hyaluronan among male, and greater shedding of syndecan CD-138 among older athletes indicate complex glycocalyx shedding during ultra-endurance exercise. This is the first study to investigate changes in glycocalyx elements in an endurance footrace and first study to investigate exercise-induced shedding in both sexes. This study comprised of an athlete group who finished the ultra-long distance of up to 690 km during the Yukon Arctic Ultra as well as a control group. Results indicate relevant and different shedding of glycocalyx elements heparan sulfate, hyaluronan, and syndecan CD-138. Sex, age, BMI, and covered distance appear to have an influence on the shedding. Other serum parameters indicative of stress appear to be associated with shedding.
内皮糖萼维持血管结构，在炎症和高强度运动期间可能会脱落。目前尚无关于超耐力运动期间糖萼脱落的研究。“育空北极超级马拉松”（Yukon Arctic Ultra，YAU）是最长和最冷的超长马拉松之一，本研究旨在探讨其对糖萼脱落的影响。13 名参加 2015-2019 年 YAU 比赛的成年人（38.92 ± 8.67 岁，6 名女性）以 4.17 ± 0.62 km/h 的移动速度完成了 657.03 ± 71.65 km。平均日温度范围为-12.6°C 至-30.5°C。在开始、277 公里、383 公里和 690 公里时，从血清中定量测定糖萼成分硫酸乙酰肝素、透明质酸和 syndecan CD-138。还定量测定了皮质醇、C 反应蛋白、肌酸激酶和脑钠肽的 N 端前体。7 名 YAU 志愿者（36.14 ± 11.04 岁，5 名女性）作为对照。对照组各时间点无变化。完成者中透明质酸显著增加，syndecan CD-138 显著减少。女性完成者在开始、383 公里和 690 公里时，硫酸乙酰肝素值更高，男性完成者在 277 公里时，透明质酸值更高。所有时间点，年龄较大的完成者 syndecan CD-138 值更高。透明质酸与距离、肌酸激酶和 NT-Pro BNP 之间存在弱显著相关性（<0.215）。YAU 参与者的糖萼成分脱落。女性硫酸乙酰肝素脱落增加，男性透明质酸增加，老年运动员 syndecan CD-138 脱落增加，表明超耐力运动中糖萼脱落复杂。这是首次研究耐力赛跑中糖萼成分的变化，也是首次研究两性运动诱导的糖萼脱落。本研究包括在育空北极超级马拉松中完成长达 690 公里超长距离的运动员组和对照组。结果表明，硫酸乙酰肝素、透明质酸和 syndecan CD-138 的糖萼成分有明显的脱落。性别、年龄、BMI 和覆盖距离似乎对脱落有影响。其他表明应激的血清参数似乎与脱落有关。
