Sjöberg A M, Alanko T A
J Assoc Off Anal Chem. 1987 May-Jun;70(3):588-90.
A spectrophotometric method for the determination of cyclamate was collaboratively studied in 9 laboratories. Ethyl acetate is added to extract cyclamate from acidic aqueous solution into water, and the cyclamate is then quantitatively converted to N,N-dichlorocyclohexylamine by adding excess hypochlorite. N,N-Dichlorocyclohexylamine is determined by measuring its UV absorption at 314 nm. Six samples, 3 soft drinks with cyclamate levels of 0.36-0.47 g/kg and 3 jams with levels of 1.23-1.50 g/kg, were included in the study. Average recoveries of cyclamate were 99.7% in the soft drinks and 103.8% in the jams. Reproducibility coefficients of variation were 6.7% for the soft drinks and 4.4% for the jams.
9个实验室协作研究了一种分光光度法测定甜蜜素。向酸性水溶液中加入乙酸乙酯以将甜蜜素萃取至水相中，然后通过加入过量次氯酸盐将甜蜜素定量转化为N,N-二氯环己胺。通过测量其在314nm处的紫外吸收来测定N,N-二氯环己胺。该研究包括6个样品，3种含甜蜜素水平为0.36 - 0.47g/kg的软饮料和3种含甜蜜素水平为1.23 - 1.50g/kg的果酱。甜蜜素在软饮料中的平均回收率为99.7%，在果酱中为103.8%。软饮料的再现性变异系数为6.7%，果酱为4.4%。
