Matsudaira P
J Biol Chem. 1987 Jul 25;262(21):10035-8.
Small amounts (7-250 pmol) of myoglobin, beta-lactoglobulin, and other proteins and peptides can be spotted or electroblotted onto polyvinylidene difluoride (PVDF) membranes, stained with Coomassie Blue, and sequenced directly. The membranes are not chemically activated or pretreated with Polybrene before usage. The average repetitive yields and initial coupling of proteins spotted or blotted into PVDF membranes ranged between 84-98% and 30-108% respectively, and were comparable with the yields measured for proteins spotted onto Polybrene-coated glass fiber discs. The results suggest that PVDF membranes are superior supports for sequence analysis of picomole quantities of proteins purified by gel electrophoresis.
少量（7 - 250皮摩尔）的肌红蛋白、β-乳球蛋白以及其他蛋白质和肽段可以点样或电印迹到聚偏二氟乙烯（PVDF）膜上，用考马斯亮蓝染色后直接进行测序。这些膜在使用前无需进行化学活化或用聚凝胺预处理。点样或印迹到PVDF膜上的蛋白质的平均重复产率和初始偶联率分别在84% - 98%和30% - 108%之间，与点样到涂有聚凝胺的玻璃纤维圆盘上的蛋白质的产率相当。结果表明，PVDF膜是对通过凝胶电泳纯化的皮摩尔量蛋白质进行序列分析的优质支持物。
