Tong Yao, Li Yong-Feng, Yi Shan-Chi, Fan Dan-Li, Qiu Zhuo-Xun, Wei Chen-Yang, Huang Meng-Ge, Zeng Dong-Qiang, Tang Wen-Wei
Guangxi Key Laboratory of Agro-Environment and Agric-Product Safety, College of Agriculture, Guangxi University, Nanning, P. R. China.
Excellence and Innovation Center, Jiangsu Academy of Agricultural Sciences, Nanjing, P. R. China.
Pest Manag Sci. 2023 Jan;79(1):163-172. doi: 10.1002/ps.7185. Epub 2022 Oct 3.
Echinochloa crus-galli var. zelayensis is a troublesome weed in rice fields and can be controlled by using quinclorac. However, over-reliance on quinclorac has resulted in resistant (R) barnyardgrass, which differs significantly in its ability to transport quinclorac compared to susceptible (S) barnyardgrass. This study aimed to investigate the underlying mechanisms for this different translocation between R and S barnyardgrass.
Larger amount of quinclorac was transferred from shoots to roots in R compared to S barnyardgrass. After 1 day of quinclorac [300 g active ingredient (a.i.) ha ] foliar treatment, its content in shoots of R was 81.92% of that in S barnyardgrass; correspondingly, in roots of R was 1.17 fold of that in S barnyardgrass. RNA-sequencing and quantitative real-time polymerase chain reaction (qRT-PCR) confirmed the expression levels of PIPs belonging to aquaporins (AQPs) in R were higher than in S barnyardgrass, with or without quinclorac treatment. With co-application of quinclorac and AQPs inhibitors [mercury(II) chloride (HgCl )] treatment, even though the expression levels of PIPs and the transport rates of quinclorac were both suppressed in R and S barnyardgrass, this process was less pronounced in R than in S barnyardgrass.
This report provides clear evidence that higher PIPs expression results in rapid quinclorac translocation from shoots to roots and reduces the quinclorac accumulation in the shoot meristems in R barnyardgrass, thus reducing the control efficacy of quinclorac. © 2022 Society of Chemical Industry.
稻稗是稻田中一种棘手的杂草，可用二氯喹啉酸进行防除。然而，过度依赖二氯喹啉酸已导致产生了对其具有抗性（R）的稗草，与敏感（S）稗草相比，抗性稗草在转运二氯喹啉酸的能力上存在显著差异。本研究旨在探究抗性和敏感稗草之间这种不同转运能力的潜在机制。
与敏感稗草相比，抗性稗草中从地上部向根部转运的二氯喹啉酸量更多。在进行二氯喹啉酸[300 g活性成分（a.i.）/公顷]叶面处理1天后，抗性稗草地上部中二氯喹啉酸的含量为敏感稗草的81.92%；相应地，抗性稗草根部中二氯喹啉酸的含量是敏感稗草的1.17倍。RNA测序和定量实时聚合酶链反应（qRT-PCR）证实，无论是否进行二氯喹啉酸处理，抗性稗草中属于水通道蛋白（AQPs）的水孔蛋白（PIPs）的表达水平均高于敏感稗草。在二氯喹啉酸与水通道蛋白抑制剂[氯化汞（HgCl₂）]共同处理时，尽管抗性和敏感稗草中PIPs的表达水平以及二氯喹啉酸的转运速率均受到抑制，但抗性稗草中的这一过程不如敏感稗草明显。
本报告提供了明确的证据，表明较高的PIPs表达导致二氯喹啉酸从地上部快速转运至根部，并减少了抗性稗草地上部顶端分生组织中二氯喹啉酸的积累，从而降低了二氯喹啉酸的防除效果。© 2022化学工业协会。
