细胞病变效应vero 细胞适应孟加拉国小反刍动物瘟病毒（PPR）株在细胞培养中。

Cytopathic effect of Vero cells adapted Bangladeshi strain of peste des petits ruminants (PPR) virus in cell culture.

机构信息

Department of Anatomy and Histology, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet, 3100, Bangladesh.

Friedrich Loffler Institut, Federal Research Institute for Animal Health, Südufer 10, 17493, Greifswald-Insel Riems, Germany.

出版信息

Arch Microbiol. 2022 Sep 16;204(10):625. doi: 10.1007/s00203-022-03239-4.

DOI:10.1007/s00203-022-03239-4

PMID:36112247

Abstract

The present study described the cytopathic effect of PPR virus presently being used in serial passages at the level of 60th in Vero cells and infected tissue culture fluid was used in this study as viral inoculum. Vero cells were grown on cover slip & were infected with tissue culture fluid at a fixed multiplicity of infection (MOI) 0.01. The infected cover slip along with control were stained with H&E stain at periodic intervals and cytopathic effect was studied with microscope. The cytopathic effect (CPE) was visible at first from 24 hpi and the Vero cells showed initial cell rounding, aggregation, and syncytial development. Development of inclusion bodies and cell degradation was noticed by 72 hpi. Complete detachment of the cell monolayer was observed by 84 hpi. It is concluded that, development of numerous inclusion bodies are the indication of well adaptation & extensive multiplication of PPRV in Vero cells.

摘要

本研究描述了在 Vero 细胞中进行第 60 代连续传代时使用的 PPR 病毒的细胞病变效应，并用感染的组织培养物作为病毒接种物。将 Vero 细胞种在盖玻片上，并以固定的感染复数（MOI）0.01 用组织培养液感染。将受感染的盖玻片与对照一起用 H&E 染色定期染色，并在显微镜下研究细胞病变效应。细胞病变效应（CPE）在 24 小时后首次可见，Vero 细胞表现出初始的细胞圆化、聚集和合胞体发育。在 72 小时后观察到包涵体的形成和细胞降解。在 84 小时后观察到细胞单层的完全脱落。结论是，大量包涵体的形成表明 PPRV 在 Vero 细胞中很好地适应和广泛增殖。