尿液中美托洛尔、氧烯洛尔和普萘洛尔对映体的快速测定
Rapid determination of the enantiomers of metoprolol, oxprenolol and propranolol in urine.
作者信息
Pflugmann G, Spahn H, Mutschler E
出版信息
J Chromatogr. 1987 May 15;416(2):331-9. doi: 10.1016/0378-4347(87)80516-9.
PMID:3611264
Abstract
A method is described that makes possible the rapid determination of the enantiomers of beta-blocking agents. After extraction from urine samples (at pH 9.9) using toluene, the enantiomers are derivatised with S-(+)-benoxaprofen chloride. The chromatographic separation can be performed on thin-layer plates with toluene-acetone as mobile phase. The derivatives can be detected by measuring the fluorescence (lambda ex = 313 nm,lambda em = 365 nm).
摘要
本文描述了一种能够快速测定β受体阻滞剂对映体的方法。从尿液样本（pH 9.9）中用甲苯萃取后，对映体用S-(+)-苯恶洛芬氯进行衍生化。色谱分离可在以甲苯 - 丙酮为流动相的薄层板上进行。衍生物可通过测量荧光（激发波长λex = 313 nm，发射波长λem = 365 nm）来检测。
Zotero 插件