利用生物素-抗生物素蛋白相互作用检测乙型肝炎表面抗原抗体（抗-HBs）。
The utilization of biotin-antibiotin interaction for the detection of antibody to hepatitis B surface antigen (anti-HBs).
作者信息
Mushahwar I K, Spiezia K S
出版信息
J Virol Methods. 1987 May;16(1-2):45-54. doi: 10.1016/0166-0934(87)90030-9.
A biotin-antibiotin solid-phase enzyme-linked immunoassay for the detection and quantitation of the antibody to hepatitis B surface antigen (anti-HBs) is described. The assay utilizes hepatitis B surface antigen as a solid-phase 'capture' reagent and a mixture of biotinylated HBsAg and antibiotin-conjugated horseradish peroxidase as a detector reagent. The assay was compared to a commercial enzyme immunoassay (AUSAB EIA) which used the biotin-avidin system for anti-HBs detection. The two assays were found to measure the same molecules and to correlate well regarding anti-HBs titers.
描述了一种用于检测和定量乙型肝炎表面抗原抗体（抗-HBs）的生物素-抗生物素固相酶联免疫测定法。该测定法利用乙型肝炎表面抗原作为固相“捕获”试剂，并使用生物素化的HBsAg和抗生物素偶联的辣根过氧化物酶的混合物作为检测试剂。将该测定法与一种使用生物素-抗生物素蛋白系统检测抗-HBs的商业酶免疫测定法（AUSAB EIA）进行了比较。发现这两种测定法测量的是相同的分子，并且在抗-HBs滴度方面具有良好的相关性。
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