Black M J, Chait L, O'Brien B M, Sykes P J, Sharzer L A
Br J Plast Surg. 1978 Oct;31(4):295-9. doi: 10.1016/s0007-1226(78)90114-5.
The supplying vessels of microvascular free flaps in pigs were ligated at various intervals after transfer in order to study the revascularisation from the recipient bed. At 8 days of later survival occurred. Earlier, although necrosis was not inevitable, the results were unpredictable.