Dept. of Molecular Biosciences, University of Texas at Austin, United States.
Dept. of Molecular Biosciences, University of Texas at Austin, United States.
Dev Biol. 2022 Nov;491:105-112. doi: 10.1016/j.ydbio.2022.08.011. Epub 2022 Sep 13.
During neural tube closure, neural ectoderm cells constrict their apical surfaces to bend and fold the tissue into a tube that will become the central nervous system. Recent data from mice and humans with neural tube defects suggest that key genes required for neural tube closure can exert non-cell autonomous effects on cell behavior, but the nature of these effects remains obscure. Here, we coupled tissue-scale, high-resolution time-lapse imaging of the closing neural tube of Xenopus to multivariate regression modeling, and we show that medial actin accumulation drives apical constriction non-autonomously in neighborhoods of cells, rather than solely in individual cells. To further explore this effect, we examined mosaic crispant embryos and identified both autonomous and non-autonomous effects of the apical constriction protein Shroom3.
在神经管闭合过程中，神经外胚层细胞收缩其顶端表面，使组织弯曲并折叠成一个将成为中枢神经系统的管状结构。来自神经管缺陷的老鼠和人类的最新数据表明，神经管闭合所需的关键基因可以对细胞行为产生非细胞自主的影响，但这些影响的性质尚不清楚。在这里，我们将爪蟾闭合神经管的组织尺度、高分辨率延时成像与多元回归建模相结合，结果表明，中膜肌动蛋白的积累而非仅在单个细胞中，驱动细胞周围的顶端收缩非自主发生。为了进一步探讨这种影响，我们检查了嵌合体脆化胚胎，并确定了顶端收缩蛋白 Shroom3 的自主和非自主效应。
