Kaneko T, Suzuki H, Takahashi T
J Dairy Sci. 1987 Jun;70(6):1128-33. doi: 10.3168/jds.S0022-0302(87)80122-4.
Acid-producing activity and viability of the liquid concentrated culture of Lactobacillus bulgaricus 1067 were markedly improved only when the cells were grown in a medium supplemented with Span 80 and then stored with .1% sodium ascorbate after addition of bubbling N2 into the culture. Oleic acid content of the cells increased from 17 to 38% to 39 to 54%, and the endothermic transition of the cell lipids shifted to a lower temperature when growth medium was supplemented with Span 80. During storage, the cells grown in the medium supplemented with Span 80 consumed a larger amount of lactose than those grown without Span 80. Also, the former cells had more ATP than the latter after storage at 5 degrees C for 14 d. Permeability of the cells for lactose might be improved by increased oleic acid content of the cellular components in which care the lactose incorporated into the cells would be utilized as the energy source during storage.
只有当保加利亚乳杆菌1067的细胞在添加了司盘80的培养基中生长，然后在向培养物中通入鼓泡氮气后添加0.1%抗坏血酸钠进行储存时，其液体浓缩培养物的产酸活性和活力才会显著提高。当生长培养基中添加司盘80时，细胞的油酸含量从17%至38%增加到39%至54%，并且细胞脂质的吸热转变温度降低。在储存期间，在添加司盘80的培养基中生长的细胞比未添加司盘80生长的细胞消耗更多的乳糖。此外，在5℃储存14天后，前者细胞比后者具有更多的ATP。细胞对乳糖的通透性可能通过细胞成分中油酸含量的增加而得到改善，在此过程中，掺入细胞中的乳糖将在储存期间用作能量来源。
