School of Materials and Environment, Guangxi Colleges and Universities Key Laboratory of Environmental-friendly Materials and New Technology for Carbon Neutralization, Guangxi Collaborative Innovation Center for Chemistry and Engineering of Forest Products, Guangxi Minzu University, Nanning 530006, China; Key Laboratory of Drinking Water Science and Technology, Research Center for Eco-environmental Sciences, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing 100085, China.
School of Materials and Environment, Guangxi Colleges and Universities Key Laboratory of Environmental-friendly Materials and New Technology for Carbon Neutralization, Guangxi Collaborative Innovation Center for Chemistry and Engineering of Forest Products, Guangxi Minzu University, Nanning 530006, China.
Sci Total Environ. 2023 Jan 15;856(Pt 1):158795. doi: 10.1016/j.scitotenv.2022.158795. Epub 2022 Sep 14.
Simultaneous removal of ammonium and nitrate was achieved in a methane-fed moving bed biofilm reactor (MBBR). In the reactor, methanotrophic microorganisms oxidized methane under hypoxic conditions likely to methanol, hence providing an electron donor to denitrifiers to reduce nitrate to nitrite that then allowed anaerobic ammonium oxidizing bacteria (Anammox) to remove excess ammonium as N. The ammonium and nitrate removal rates reached 72.09 ± 5.81 mgNH-N/L/d and 62.61 ± 4.17 mgNO-N/L/d when the MBBR was operated in continuous mode. Nitrate removal by the methane-fed mixed consortia was confirmed in a batch test revealing a CH/NO molar removal ratio of 1.15. The functional populations were unveiled by FISH analysis and 16S rRNA gene sequencing, which showed that the biofilm was dominated by Anammox bacteria (Candidatus Kuenenia) and diverse taxa associated with the capacity for denitrification: aerobic methanotrophs (Methylobacter, Methylomonas, and unclassified Methylococcaceae), methylotrophic denitrifiers (Opitutaceae and Methylophilaceae), and other heterotrophic denitrifiers (Ignavibacteriaceae, Anaerolineaceae, Comamonadaceae, Rhodocyclaceae and Thauera). Neither DAMO archaea nor DAMO bacteria were found in the sequencing analysis, indicating that more unknown community members possess the metabolic capacity of methanotrophic denitrification.
在甲烷进料移动床生物膜反应器 (MBBR) 中实现了铵和硝酸盐的同时去除。在该反应器中，甲烷营养型微生物在缺氧条件下将甲烷氧化为甲醇，从而为反硝化菌提供电子供体，将硝酸盐还原为亚硝酸盐，然后允许厌氧氨氧化菌 (Anammox) 将过量的铵转化为 N 去除。当 MBBR 以连续模式运行时，铵和硝酸盐的去除率分别达到 72.09 ± 5.81 mgNH-N/L/d 和 62.61 ± 4.17 mgNO-N/L/d。在批处理试验中证实了甲烷进料混合菌群对硝酸盐的去除作用，表明 CH/NO 摩尔去除比为 1.15。通过荧光原位杂交 (FISH) 分析和 16S rRNA 基因测序揭示了功能菌群，结果表明生物膜主要由厌氧氨氧化菌 (Candidatus Kuenenia) 和与反硝化能力相关的多种类群组成：好氧甲烷营养菌 (Methylobacter、Methylomonas 和未分类的 Methylococcaceae)、甲基营养型反硝化菌 (Opitutaceae 和 Methylophilaceae) 和其他异养反硝化菌 (Ignavibacteriaceae、Anaerolineaceae、Comamonadaceae、Rhodocyclaceae 和 Thauera)。测序分析中未发现 DAMO 古菌或 DAMO 细菌，这表明更多未知的群落成员具有甲烷营养型反硝化的代谢能力。
