Department of General Surgery, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
Shanghai Minimally Invasive Surgery Center, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
Front Immunol. 2022 Sep 2;13:1008975. doi: 10.3389/fimmu.2022.1008975. eCollection 2022.
Colorectal cancer (CRC) is the third most common form of cancer, and the incidence of sporadic young-onset colorectal cancer (yCRC) has been increasing. Microbiota residing in the tumor microenvironment are emerging tumor components. The colonic microbiome differs between patients with CRC and healthy controls; however, few studies have investigated the role of the tumor microbiota in disease diagnosis and tumorigenesis of yCRC. We performed 16S rRNA sequencing analysis to identify the microbiome in CRC and found that tumor microbial diversity decreased in yCRC. Proteobacteria and Firmicutes were the most abundant phyla in all CRC samples, and and were the key microbiota in the yCRC group. Correlation analysis revealed that co-occurred with various pro-tumor microbial taxa, including Bacteroidia, Gammaproteobacteria, and . An independent cohort was used to validate the results. The in CRC was co-localized with cancer-associated fibroblasts and activated the TLR2/NF-κB pathway and reduces CD8+ T lymphocyte infiltration in CRC microenvironment. This study suggests that tumoral microbiota plays an important role in promoting tumorigenesis and therefore has potential as a promising non-invasive tool and intervention target for anti-tumor therapy.
结直肠癌（CRC）是第三大常见癌症，散发性早发性结直肠癌（yCRC）的发病率一直在增加。驻留在肿瘤微环境中的微生物群是新兴的肿瘤成分。CRC 患者和健康对照者的结肠微生物群不同；然而，很少有研究调查肿瘤微生物群在 yCRC 疾病诊断和肿瘤发生中的作用。我们进行了 16S rRNA 测序分析，以鉴定 CRC 中的微生物组，发现 yCRC 中的肿瘤微生物多样性降低。变形菌门和厚壁菌门是所有 CRC 样本中最丰富的门，和 是 yCRC 组中的关键微生物群。相关性分析表明 与各种促肿瘤微生物类群共同存在，包括拟杆菌门、γ-变形菌门和。使用独立队列验证了结果。CRC 中的 与癌症相关成纤维细胞共定位，并激活 TLR2/NF-κB 通路，减少 CRC 微环境中 CD8+T 淋巴细胞浸润。这项研究表明，肿瘤微生物群在促进肿瘤发生中起着重要作用，因此具有作为有前途的非侵入性工具和抗肿瘤治疗干预靶点的潜力。
