Buccione R, Cecconi S, Tatone C, Mangia F, Colonna R
J Exp Zool. 1987 Jun;242(3):351-4. doi: 10.1002/jez.1402420314.
To investigate mechanisms of follicle cell control on mammalian oocyte growth, preantral mouse oocytes free from surrounding follicle cells were individually cocultured with monolayers of different somatic cells competent to form gap junctions, and the rate of in vitro oocyte growth was directly correlated with the level of metabolic coupling on the same cells. The results indicate that 1) at a similar extent of metabolic coupling, mouse oocytes grew on follicle cells but not on 3T3 and Sertoli cell monolayers, and 2) the growth rate of oocytes cultured on follicle cells was dependent on the extent of metabolic coupling. It was concluded that gap-junction-mediated nutrition of ovarian mouse oocytes exerted by somatic cells is necessary but not sufficient to maintain oocyte growth. A specific regulatory role of follicle cells on mammalian oocyte growth is proposed.
为了研究卵泡细胞对哺乳动物卵母细胞生长的控制机制，将去除周围卵泡细胞的小鼠窦前卵母细胞分别与能够形成缝隙连接的不同体细胞单层进行共培养，并且体外卵母细胞的生长速率与相同细胞上的代谢偶联水平直接相关。结果表明：1）在相似的代谢偶联程度下，小鼠卵母细胞在卵泡细胞上生长，但不在3T3和支持细胞单层上生长；2）在卵泡细胞上培养的卵母细胞的生长速率取决于代谢偶联的程度。得出的结论是，体细胞通过缝隙连接介导的对卵巢小鼠卵母细胞的营养作用是维持卵母细胞生长所必需的，但并不充分。提出了卵泡细胞对哺乳动物卵母细胞生长的特定调节作用。
