用氨甲喋呤和中波紫外线对三倍剂量的单采血小板进行病原体灭活处理。
Pathogen inactivation treatment of triple-dose apheresis platelets with amotosalen and ultraviolet a light.
机构信息
Regional Blood Transfusion Service, Swiss Red Cross, Division of Hematology, University Hospital, Basel, Switzerland.
University of Basel, Basel, Switzerland.
出版信息
Transfus Med. 2022 Dec;32(6):505-511. doi: 10.1111/tme.12913. Epub 2022 Sep 20.
BACKGROUND
A triple storage (TS) set allows for pathogen inactivation (PI) treatment of triple-dose apheresis platelet products with amotosalen + UVA. We evaluated the quality and metabolic parameters of platelet concentrates (PCs) pathogen inactivated and stored for 7 days.
MATERIALS AND METHODS
Twelve triple-dose products collected with two different apheresis platforms were treated with amotosalen+UVA. Products were split into three single-dose units. Testing was made pretreatment, after splitting, at days 5 and 7 of storage.
RESULTS
Single-dose PI PCs had a mean platelet content of 2.89 ± 0.35 x 10 . From baseline to day 7, pH remained stable (7.1 ± 0.1 vs. 7.0 ± 0.1), pO increased (11.3 ± 2.4 vs. 18.3 ± 3.5 kPa) as did LDH (201 ± 119 vs. 324 ± 203 U/L) and lactate (3.6 ± 1.7 vs. 12.1 ± 1.5 mmol/L) (all p < 0.01); pCO decreased (4.1 ± 0.8 vs. 1.5 ± 0.7 mmHg; p < 0.01) and so did bicarbonate (6.6 ± 1.1 vs. 2.5 ± 1.4 mmol/L), glucose (5.6 ± 1.2 vs. 0.4 ± 0.4 mmol/L) and ATP (3.4 ± 0.9 vs. 2.5 ± 1.4 nmol/10 platelets) (all p < 0.05).
CONCLUSION
Triple-dose PCs processed with the TS sets fulfilled the quality requirements and displayed metabolic changes of expected extent during 7-day storage.
背景
三重储存（TS）套装可用于对三剂量单采血小板产品进行病原体灭活（PI）处理，采用氨甲喋呤+UVA。我们评估了经过 PI 处理并储存 7 天的血小板浓缩物（PC）的质量和代谢参数。
材料和方法
用两种不同的单采平台采集了 12 个三剂量产品，用氨甲喋呤+UVA 进行处理。将产品分为三个单剂量单位。在预处理、分切后，以及储存第 5 和 7 天进行测试。
结果
单剂量 PI-PC 的平均血小板含量为 2.89±0.35×1011/L。从基线到第 7 天，pH 值保持稳定（7.1±0.1 与 7.0±0.1），pO2 增加（11.3±2.4 与 18.3±3.5 kPa），LDH（201±119 与 324±203 U/L）和乳酸（3.6±1.7 与 12.1±1.5 mmol/L）也升高（均 p<0.01）；pCO2 降低（4.1±0.8 与 1.5±0.7 mmHg；p<0.01），碳酸氢盐（6.6±1.1 与 2.5±1.4 mmol/L）、葡萄糖（5.6±1.2 与 0.4±0.4 mmol/L）和 ATP（3.4±0.9 与 2.5±1.4 nmol/10 血小板）也降低（均 p<0.05）。
结论
用 TS 套装处理的三剂量 PC 在 7 天储存期内满足质量要求，并显示出预期程度的代谢变化。
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