Regional Blood Transfusion Service, Swiss Red Cross, Division of Hematology, University Hospital, Basel, Switzerland.
University of Basel, Basel, Switzerland.
Transfus Med. 2022 Dec;32(6):505-511. doi: 10.1111/tme.12913. Epub 2022 Sep 20.
A triple storage (TS) set allows for pathogen inactivation (PI) treatment of triple-dose apheresis platelet products with amotosalen + UVA. We evaluated the quality and metabolic parameters of platelet concentrates (PCs) pathogen inactivated and stored for 7 days.
Twelve triple-dose products collected with two different apheresis platforms were treated with amotosalen+UVA. Products were split into three single-dose units. Testing was made pretreatment, after splitting, at days 5 and 7 of storage.
Single-dose PI PCs had a mean platelet content of 2.89 ± 0.35 x 10 . From baseline to day 7, pH remained stable (7.1 ± 0.1 vs. 7.0 ± 0.1), pO increased (11.3 ± 2.4 vs. 18.3 ± 3.5 kPa) as did LDH (201 ± 119 vs. 324 ± 203 U/L) and lactate (3.6 ± 1.7 vs. 12.1 ± 1.5 mmol/L) (all p < 0.01); pCO decreased (4.1 ± 0.8 vs. 1.5 ± 0.7 mmHg; p < 0.01) and so did bicarbonate (6.6 ± 1.1 vs. 2.5 ± 1.4 mmol/L), glucose (5.6 ± 1.2 vs. 0.4 ± 0.4 mmol/L) and ATP (3.4 ± 0.9 vs. 2.5 ± 1.4 nmol/10 platelets) (all p < 0.05).
Triple-dose PCs processed with the TS sets fulfilled the quality requirements and displayed metabolic changes of expected extent during 7-day storage.
三重储存（TS）套装可用于对三剂量单采血小板产品进行病原体灭活（PI）处理，采用氨甲喋呤+UVA。我们评估了经过 PI 处理并储存 7 天的血小板浓缩物（PC）的质量和代谢参数。
用两种不同的单采平台采集了 12 个三剂量产品，用氨甲喋呤+UVA 进行处理。将产品分为三个单剂量单位。在预处理、分切后，以及储存第 5 和 7 天进行测试。
单剂量 PI-PC 的平均血小板含量为 2.89±0.35×1011/L。从基线到第 7 天，pH 值保持稳定（7.1±0.1 与 7.0±0.1），pO2 增加（11.3±2.4 与 18.3±3.5 kPa），LDH（201±119 与 324±203 U/L）和乳酸（3.6±1.7 与 12.1±1.5 mmol/L）也升高（均 p<0.01）；pCO2 降低（4.1±0.8 与 1.5±0.7 mmHg；p<0.01），碳酸氢盐（6.6±1.1 与 2.5±1.4 mmol/L）、葡萄糖（5.6±1.2 与 0.4±0.4 mmol/L）和 ATP（3.4±0.9 与 2.5±1.4 nmol/10 血小板）也降低（均 p<0.05）。
用 TS 套装处理的三剂量 PC 在 7 天储存期内满足质量要求，并显示出预期程度的代谢变化。
