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从哥斯达黎加一份牛脑样本中分离出的水疱性口炎病毒。

Vesicular Stomatitis Virus Isolated from a Bovine Brain Sample in Costa Rica.

作者信息

León Bernal, Cordero-Solorzano Juan M, Rodríguez Luis, Jiménez Carlos

机构信息

Biosecurity Laboratory (LSE), Animal Health National Service (SENASA), Heredia, Costa Rica.

Universidad Técnica Nacional (UTN), Atenas, Costa Rica.

出版信息

Microbiol Resour Announc. 2022 Oct 20;11(10):e0073722. doi: 10.1128/mra.00737-22. Epub 2022 Sep 20.

DOI:10.1128/mra.00737-22
PMID:36125285
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9584207/
Abstract

The genome of Vesicular stomatitis (New Jersey) virus was obtained by high-throughput sequencing after the nucleic acid was extracted from the supernatant of Vero E6 cells inoculated with a sample of a bovine brain with nervous signs. The sample was negative for rabies by direct Immunofluorescence and bovine spongiform encephalopathy by histopathology.

摘要

从出现神经症状的牛脑样本接种的Vero E6细胞上清液中提取核酸后，通过高通量测序获得了水疱性口炎（新泽西型）病毒的基因组。该样本经直接免疫荧光检测狂犬病呈阴性，经组织病理学检测牛海绵状脑病呈阴性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8863/9584207/584b86855886/mra.00737-22-f001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8863/9584207/584b86855886/mra.00737-22-f001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8863/9584207/584b86855886/mra.00737-22-f001.jpg

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本文引用的文献

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First Complete Coding Sequence of a Venezuelan Equine Encephalitis Virus Strain Isolated from an Equine Encephalitis Case in Costa Rica.从哥斯达黎加一例马脑炎病例中分离出的委内瑞拉马脑炎病毒株的首个完整编码序列。
Microbiol Resour Announc. 2019 Sep 5;8(36):e00672-19. doi: 10.1128/MRA.00672-19.
2
Genome Detective: an automated system for virus identification from high-throughput sequencing data.基因组侦探：一种从高通量测序数据中自动识别病毒的系统。
Bioinformatics. 2019 Mar 1;35(5):871-873. doi: 10.1093/bioinformatics/bty695.
3
MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms.
MEGA X：跨越计算平台的分子进化遗传学分析。
Mol Biol Evol. 2018 Jun 1;35(6):1547-1549. doi: 10.1093/molbev/msy096.
4
Gene-wide identification of episodic selection.全基因范围内间歇性选择的鉴定。
Mol Biol Evol. 2015 May;32(5):1365-71. doi: 10.1093/molbev/msv035. Epub 2015 Feb 19.
5
Less is more: an adaptive branch-site random effects model for efficient detection of episodic diversifying selection.少即是多：一种用于有效检测 episodic 多样化选择的适应性分支位点随机效应模型。
Mol Biol Evol. 2015 May;32(5):1342-53. doi: 10.1093/molbev/msv022. Epub 2015 Feb 19.
6
Full-length genome analysis of vesicular stomatitis New Jersey virus strains representing the phylogenetic and geographic diversity of the virus.代表水疱性口炎新泽西病毒系统发育和地理多样性的病毒株的全基因组分析。
Arch Virol. 2012 Nov;157(11):2247-51. doi: 10.1007/s00705-012-1420-x. Epub 2012 Jul 24.
7
Genetic and antigenic relationships of vesicular stomatitis viruses from South America.南美洲口蹄疫病毒的遗传和抗原关系。
Arch Virol. 2011 Nov;156(11):1961-8. doi: 10.1007/s00705-011-1081-1. Epub 2011 Aug 10.
8
Purification of RNA using TRIzol (TRI reagent).使用TRIzol（TRI试剂）纯化RNA。
Cold Spring Harb Protoc. 2010 Jun;2010(6):pdb.prot5439. doi: 10.1101/pdb.prot5439.
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Vesicular stomatitis virus infects resident cells of the central nervous system and induces replication-dependent inflammatory responses.水疱性口炎病毒感染中枢神经系统的固有细胞，并诱导依赖复制的炎症反应。
Virology. 2010 May 10;400(2):187-96. doi: 10.1016/j.virol.2010.01.025. Epub 2010 Feb 20.
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Spatial and phylogenetic analysis of vesicular stomatitis virus over-wintering in the United States.美国越冬水疱性口炎病毒的时空和系统发育分析。
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