León Bernal, Cordero-Solorzano Juan M, Rodríguez Luis, Jiménez Carlos
Biosecurity Laboratory (LSE), Animal Health National Service (SENASA), Heredia, Costa Rica.
Universidad Técnica Nacional (UTN), Atenas, Costa Rica.
Microbiol Resour Announc. 2022 Oct 20;11(10):e0073722. doi: 10.1128/mra.00737-22. Epub 2022 Sep 20.
The genome of Vesicular stomatitis (New Jersey) virus was obtained by high-throughput sequencing after the nucleic acid was extracted from the supernatant of Vero E6 cells inoculated with a sample of a bovine brain with nervous signs. The sample was negative for rabies by direct Immunofluorescence and bovine spongiform encephalopathy by histopathology.
从出现神经症状的牛脑样本接种的Vero E6细胞上清液中提取核酸后,通过高通量测序获得了水疱性口炎(新泽西型)病毒的基因组。该样本经直接免疫荧光检测狂犬病呈阴性,经组织病理学检测牛海绵状脑病呈阴性。