Department of Chemistry, Isfahan University of Technology, Isfahan, 84156-83111, Iran.
Department of Chemistry, Shahreza Branch, Islamic Azad University, P.O. Box 311-86145, Shahreza, Isfahan, Iran.
Anal Sci. 2022 Dec;38(12):1533-1540. doi: 10.1007/s44211-022-00188-2. Epub 2022 Sep 20.
In this work, dispersive liquid-liquid microextraction (DLLME) based on high-density extraction solvent was applied as a simple, fast and sensitive method for extraction and preconcentration of methamphetamine from human plasma and urine samples. The efficiency of positive corona discharge ionization ion mobility spectrometry was investigated for direct analysis of the extracted analyte. Effective parameters on the extraction efficiency, such as type and volume of the extraction and disperser solvents, centrifugation time, and sample solution pH were optimized. Trichloromethane and isopropanol were selected as the extracting and disperser solvents, respectively. Under the optimized conditions, the linear dynamic range (R = 0.9969) was found to be 0.5-18 µg/L, and 0.15 µg/L was calculated as the limit of detection. The relative standard deviations of intra- and inter-day were obtained 4 and 10%, respectively, and finally, in the analysis of human plasma and urine samples, the extraction recovery was obtained 104%.
在这项工作中，采用基于高密度萃取溶剂的分散液-液微萃取（DLLME）作为一种简单、快速、灵敏的方法，从人血浆和尿液样品中萃取和预浓缩苯丙胺。考察了正电晕放电电离离子淌度谱对萃取分析物的直接分析效率。优化了萃取效率的有效参数，如萃取和分散溶剂的类型和体积、离心时间和样品溶液 pH 值。三氯甲烷和异丙醇分别选为萃取和分散溶剂。在优化条件下，线性动态范围（R=0.9969）为 0.5-18μg/L，检测限为 0.15μg/L。日内和日间相对标准偏差分别为 4%和 10%，最后，在人血浆和尿液样品分析中，萃取回收率为 104%。
