School of Horticulture and Plant Protection, Yangzhou University, Yangzhou, Jiangsu 225009, China; Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, Yangzhou, Jiangsu 225009, China.
School of Food Science and Engineering, Yangzhou University, Yangzhou, Jiangsu 225009, China.
Pestic Biochem Physiol. 2022 Oct;187:105194. doi: 10.1016/j.pestbp.2022.105194. Epub 2022 Aug 4.
Chlorantraniliprole (CAP), a representative bisamide insecticide, is widely used in rice fields around the world, posing potential toxicity risks to aquatic organisms. In this study, we examined the effects of exposure to CAP on growth and metabolic phenotype of zebrafish (Danio rerio) and oxidative stress and apoptosis in the liver of zebrafish (Danio rerio). First, we identified that CAP had a low bioaccumulation in zebrafish. Subsequently, growth phenotype analysis revealed that CAP could significantly increase liver weight and liver index in zebrafish. In addition, we found that CAP exposure could cause significant changes in indicators of oxidative stress, resulting in a significant increase in the content of malondialdehyde (MDA), causing oxidative stress in the liver of zebrafish. Meanwhile, the expression levels of apoptosis-related genes were also significantly changed and apoptosis was promoted in the liver of zebrafish with CAP exposure. Importantly, the results of metabolomics analysis shown that CAP exposure could significantly disrupt the metabolic phenotype of zebrafish, interfering with multiple metabolic pathways, mainly including valine, leucine and isoleucine biosynthesis and degradation, alanine, aspartate and glutamate metabolism and d-glutamine and D-glutamate metabolism. Last but not least, correlation analysis identified strong links between changes in liver function involving oxidative stress and apoptosis and changes in metabolic phenotype of zebrafish following CAP exposure. In brief, these results indicate that potential environmental risks of CAP to aquatic organisms should receive more attention.
氯虫苯甲酰胺（CAP）是一种代表性的双酰胺类杀虫剂，广泛应用于世界各地的稻田，对水生生物具有潜在的毒性风险。在这项研究中，我们研究了 CAP 暴露对斑马鱼（Danio rerio）生长和代谢表型以及斑马鱼（Danio rerio）肝脏氧化应激和细胞凋亡的影响。首先，我们发现 CAP 在斑马鱼体内的生物蓄积性较低。随后，生长表型分析表明，CAP 可显著增加斑马鱼的肝脏重量和肝脏指数。此外，我们发现 CAP 暴露可导致氧化应激指标发生显著变化，导致丙二醛（MDA）含量显著增加，引起斑马鱼肝脏氧化应激。同时，凋亡相关基因的表达水平也发生了显著变化，CAP 暴露促进了斑马鱼肝脏的细胞凋亡。重要的是，代谢组学分析的结果表明，CAP 暴露可显著破坏斑马鱼的代谢表型，干扰多个代谢途径，主要包括缬氨酸、亮氨酸和异亮氨酸的生物合成和降解、丙氨酸、天冬氨酸和谷氨酸代谢以及 D-谷氨酰胺和 D-谷氨酸代谢。最后但同样重要的是，相关性分析表明，CAP 暴露后斑马鱼肝脏氧化应激和细胞凋亡相关的肝功能变化与代谢表型的变化之间存在很强的关联。总之，这些结果表明，CAP 对水生生物的潜在环境风险应引起更多关注。
