Zhu Hongyuan, Guan Xianjun, Pu Lei, Shen Liyang, Hua Hongxia
Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
Pest Manag Sci. 2023 Jan;79(1):206-215. doi: 10.1002/ps.7189. Epub 2022 Oct 13.
Pesticides are extensively applied globally. Pesticide residues induce calamitous effects on the environment and untargeted organisms. Public concerns for the safety of freshwater organisms and the challenges posed by aquatic contaminants remain high. In the present study, the acute toxicity of avermectins (AVMs) to the crayfish, Procambarus clarkii was evaluated. We also evaluated the potential effects of AVM on the biochemical and transcriptomic status of the hepatopancreas and gastrointestinal tract in P. clarkii.
The 24, 48, 72, 96 h median lethal concentrations (LC ) of AVM on crayfish were 2.626, 1.162, 0.723, 0.566 mg L , respectively. The crayfish were then exposed to 0.65 mg L of AVM for 96 h. AVM significantly altered biochemical parameters including AChE and CAT activities in the hepatopancreas, and AChE, SOD and Na + -K + -ATPase activities in the gastrointestinal tract at several time points. Furthermore, transcriptomic analysis identified 953 and 1851 differentially-expressed genes (DEGs) in the hepatopancreas and gastrointestinal tract, respectively. KEGG enrichment showed that the gene expression profiles of the hepatopancreas and gastrointestinal tract were distinct from each other. The DEGs in the hepatopancreas were mostly enriched with stress-response pathways, while the majority of the DEGs in the gastrointestinal tract belonged to metabolism-related pathways.
We demonstrated that the AVM induced acute toxicity, oxidative stress, osmoregulation disturbance, neurotoxicity and transcriptome imbalance in crayfish. These findings unraveled the detrimental effects of AVMs exposure on crayfish. © 2022 Society of Chemical Industry.
农药在全球范围内广泛使用。农药残留会对环境和非目标生物造成灾难性影响。公众对淡水生物安全以及水生污染物带来的挑战的关注度仍然很高。在本研究中，评估了阿维菌素（AVM）对克氏原螯虾的急性毒性。我们还评估了AVM对克氏原螯虾肝胰腺和胃肠道生化及转录组状态的潜在影响。
AVM对克氏原螯虾的24、48、72、96小时半数致死浓度（LC）分别为2.626、1.162、0.723、0.566mg/L。然后将克氏原螯虾暴露于0.65mg/L的AVM中96小时。在几个时间点，AVM显著改变了生化参数，包括肝胰腺中的乙酰胆碱酯酶（AChE）和过氧化氢酶（CAT）活性，以及胃肠道中的AChE、超氧化物歧化酶（SOD）和钠钾ATP酶活性。此外，转录组分析分别在肝胰腺和胃肠道中鉴定出953个和1851个差异表达基因（DEG）。京都基因与基因组百科全书（KEGG）富集分析表明，肝胰腺和胃肠道的基因表达谱彼此不同。肝胰腺中的DEG大多富集于应激反应途径，而胃肠道中的大多数DEG属于代谢相关途径。
我们证明了AVM会在克氏原螯虾中诱导急性毒性、氧化应激、渗透调节紊乱、神经毒性和转录组失衡。这些发现揭示了暴露于AVM对克氏原螯虾的有害影响。©2022化学工业协会。
