Antibiotics Research and Re-evaluation Key Laboratory of Sichuan Province, School of Pharmacy, Chengdu University, Chengdu 610106, PR China.
College of Biotechnology Engineering, Sichuan University of Science & Engineering, Zigong 643000, PR China.
Int J Syst Evol Microbiol. 2022 Sep;72(9). doi: 10.1099/ijsem.0.005526.
A novel actinobacterium, designated strain H8750, was isolated from sediment sampled at Lugu Lake, southwest PR China and its polyphasic taxonomy was studied. Strain H8750 produced well-developed substrate mycelium, and formed club-shaped and hooked structures borne on the tip of the aerial mycelia. It contained -diaminopimelic, glucose, ribose and madurose in whole-cell hydrolysates. The predominant menaquinones were MK-9(H), MK-9(H) and MK-9(H). The phospholipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, unidentified phospholipids and unidentified aminophospholipids. The major fatty acid (>10 %) were 9 C, iso-C and C. The DNA G+C content was 69.7 mol% based on the whole genome sequence. Phylogenetic analysis based on 16S rRNA gene and whole-genome sequences indicated that strain H8750 was closely related to JCM 10982 (98.0 %), JCM 10983 (97.9 %) and DSM 45867 (97.8 %) and formed a monophyletic clade within the genus in the phylogenetic trees. The average nucleotide identity and digital DNA-DNA hybridization values between strain H8750 and its closely related species were 79.887.2 % and 25.928.0 %, respectively, which showed that it belonged to a distinct species. Furthermore, the morphological and phenotypic characteristics allowed the isolate to be differentiated from its closely related species. Therefore, it is concluded that strain H8750 can be classified as representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is H8750 (=JCM 34849=CICC 25116). Moreover, based on the gene prediction results, strain H8750 may have the genetic potential to synthesize many new secondary metabolites, which further increases its bioactive value.
一株新的放线菌，命名为 H8750 菌株，从中国西南部泸沽湖的沉积物中分离得到，并对其进行了多相分类学研究。菌株 H8750 产生发育良好的基质菌丝，并在气生菌丝的顶端形成棒状和钩状结构。其全细胞水解物中含有 -二氨基庚二酸、葡萄糖、核糖和马杜糖。主要的类异戊二烯醌为 MK-9(H)、MK-9(H)和 MK-9(H)。磷脂图谱含有双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、羟基磷脂酰乙醇胺、未鉴定的磷脂和未鉴定的氨基磷脂。主要脂肪酸 (>10 %)为 9C、iso-C 和 C。基于全基因组序列，DNA G+C 含量为 69.7mol%。基于 16S rRNA 基因和全基因组序列的系统发育分析表明，菌株 H8750 与 JCM 10982(98.0%)、JCM 10983(97.9%)和 DSM 45867(97.8%)密切相关，并在系统发育树中形成一个单系分支。与密切相关的物种相比，菌株 H8750 的平均核苷酸身份和数字 DNA-DNA 杂交值分别为 79.8%87.2%和 25.9%28.0%，表明其属于一个独特的物种。此外，形态和表型特征使该分离株能够与密切相关的物种区分开来。因此，推断菌株 H8750 可归类为代表 属的一个新种，提议将其命名为 sp. nov.。其模式菌株为 H8750(=JCM 34849=CICC 25116)。此外，基于基因预测结果，菌株 H8750 可能具有合成许多新次生代谢产物的遗传潜力，这进一步提高了其生物活性价值。
