Lv Feng, Gong Yuzhu, Cao Yingying, Deng Yaoyao, Liang Shufeng, Tian Xin, Gu Hongwei, Yin Jun-Jie
College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Suzhou Nano Science and Technology, Collaborative Innovation Center for New-type Urbanization and Social Governance of Jiangsu Province, Soochow University Suzhou 215123 P. R. China
School of Chemical Engineering and Materials, Changzhou Institute of Technology Changzhou 213032 P. R. China.
Nanoscale Adv. 2020 Feb 27;2(4):1583-1589. doi: 10.1039/d0na00002g. eCollection 2020 Apr 15.
Effective alcohol detection represents a substantial concern not only in the context of personal and automobile safety but also in clinical settings as alcohol is a contributing factor in a wide range of health complications including various types of liver cirrhoses, strokes, and cardiovascular diseases. Recently, many kinds of nanomaterials with enzyme-like properties have been widely used as biosensors. Herein, we have developed a convenient detection method that combines Au@PtRu nanozymes and alcohol oxidase (AOx). We found that the Au@PtRu nanorods exhibited peroxidase-like catalytic activity that was much higher than the catalytic activities of the Au and Au@Pt nanorods. The Au@PtRu nanorod-catalyzed generation of hydroxyl radicals in the presence of HO was used to develop an alcohol sensor by monitoring the HO formed by the oxidation of alcohol to acetaldehyde in the presence of AOx. When coupled with AOx, alcohol was detected down to 23.8 μM in a buffer solution for biological assays. Notably, alcohol was successfully detected in mouse blood samples with results comparable to that from commercial alcohol meters. These results highlight the potential of the Au@PtRu nanorods with peroxidase-like activity for alcohol detection, which opens up a new avenue for nanozyme development for biomedical applications.
有效的酒精检测不仅在个人和汽车安全方面是一个重大问题，在临床环境中也是如此，因为酒精是多种健康并发症的一个促成因素，包括各种类型的肝硬化、中风和心血管疾病。最近，许多具有类酶性质的纳米材料已被广泛用作生物传感器。在此，我们开发了一种将Au@PtRu纳米酶与酒精氧化酶（AOx）相结合的便捷检测方法。我们发现Au@PtRu纳米棒表现出类似过氧化物酶的催化活性，其活性远高于Au和Au@Pt纳米棒的催化活性。在HO存在下，Au@PtRu纳米棒催化产生羟基自由基，通过监测在AOx存在下酒精氧化为乙醛过程中形成的HO来开发酒精传感器。与AOx结合使用时，在用于生物测定的缓冲溶液中可检测到低至23.8 μM的酒精。值得注意的是，在小鼠血液样本中成功检测到了酒精，结果与商用酒精计相当。这些结果突出了具有类似过氧化物酶活性的Au@PtRu纳米棒在酒精检测方面的潜力，为生物医学应用的纳米酶开发开辟了一条新途径。
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