Wei Dali, Zhang Xuyun, Chen Bin, Zeng Kun
School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang, Jiangsu, 212013, China; College of Environmental Science and Engineering, Tongji University, Shanghai, 200092, China.
School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang, Jiangsu, 212013, China.
Anal Chim Acta. 2020 Aug 22;1126:106-113. doi: 10.1016/j.aca.2020.06.009. Epub 2020 Jun 28.
Because of the advantages of simplicity, cost-effectiveness and visibility, lateral-flow immunoassays (LFAs) have been widely used in the food safety field. However, the low sensitivity of LFAs needs to be solved. Nanozymes have amazing potential for application in biosensors due to their excellent and abundant enzyme-like characteristics. In this study, an Au@Pt nanozyme synthesized by a one-step method showed the higher affinity with TMB/HO and higher catalytic efficiency than that of horseradish peroxidase (HRP). For the detection of streptomycin (STR), a typical aminoglycoside antibiotic, a novel LFA based on Au@Pt as a visual tag and an enhanced LFA based on the enzyme-like activity of Au@Pt by addition of the chromogenic substrate 3-amino-9-ethyl-carbazole (AEC) were established and compared with conventional LFA based on AuNPs. The qualitative limit of detection (LOD) was 1 ng mL for the LFA based on Au@Pt as the visual tag and 0.1 ng mL for the enhanced LFA based on the activity of Au@Pt, in comparison to 8 ng mL for LFA based on AuNPs. Furthermore, the level of streptomycin in milk samples from Zhenjiang City was successfully evaluated by the novel LFA based on Au@Pt nanozyme. These results suggest that LFAs based on nanozymes are a promising and effective tool for food safety.
由于具有操作简单、成本效益高和可视化等优点，侧向流动免疫分析（LFA）已在食品安全领域得到广泛应用。然而，LFA的低灵敏度问题仍有待解决。纳米酶因其优异且丰富的类酶特性，在生物传感器领域具有惊人的应用潜力。在本研究中，通过一步法合成的Au@Pt纳米酶与TMB/HO具有更高的亲和力，且催化效率高于辣根过氧化物酶（HRP）。针对典型氨基糖苷类抗生素链霉素（STR）的检测，建立了基于Au@Pt作为可视化标签的新型LFA以及通过添加显色底物3-氨基-9-乙基咔唑（AEC）基于Au@Pt类酶活性的增强型LFA，并与基于金纳米颗粒（AuNPs）的传统LFA进行比较。基于Au@Pt作为可视化标签的LFA定性检测限为1 ng/mL，基于Au@Pt活性的增强型LFA定性检测限为0.1 ng/mL，而基于AuNPs的LFA定性检测限为8 ng/mL。此外，基于Au@Pt纳米酶的新型LFA成功评估了镇江市牛奶样品中的链霉素含量。这些结果表明，基于纳米酶的LFA是食品安全领域一种有前景且有效的工具。
