A simplified cannulation procedure for pharmacokinetic experiments in rats.

A convenient, simplified method for cannulating the jugular vein of rats is described. The technique has been developed for use in pharmacokinetic experiments. The construction of the cannula has been simplified compared with previously described methods. In addition, a small and simple outlet device for the cannula is described which allows the connection of tubing for sampling at short time intervals or for short-term infusions (several hours). In order to reduce stress on the animal, the tubing can be disconnected during sampling at long time intervals, allowing free movement of the animal with only a very small external outlet device.