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以日本海褐藻提取物与果汁和浆果汁为原料的即饮型功能性饮品

[Instant drinks based on extracts of Japan sea brown algae and fruit and berry juices as functional products].

作者信息

Tabakaev A V, Tabakaeva O V

机构信息

Far Eastern Federal University, Institute of Life Sciences and Biomedicine, 690920, Vladivostok, Russian Island, Ajax, Russian Federation.

出版信息

Vopr Pitan. 2022;91(4):107-114. doi: 10.33029/0042-8833-2022-91-4-107-114. Epub 2022 Jul 1.

DOI:10.33029/0042-8833-2022-91-4-107-114
PMID:36136952
Abstract

Nowadays, there is a need to create functional drinks that not only normalize the water-electrolyte balance, but also correct and optimize the chemical structure of the diet. The basis for the production of dry drinks can be fruit and berry juices, extracts of herbal raw materials, including algae, etc. of the study was to develop dry drinks based on dry extracts of brown algae (Costaria costata and Undaria pinnatifida) and concentrated fruit and berry juices and to evaluate the content of biologically active substances in them, as well as the antioxidant properties of the resulting drinks. . Brown algae of the Far Eastern region Costaria costata and Undaria pinnatifida were used as objects, from which dry hydrothermal extracts were obtained, as well as dry drinks based on these extracts (21-26%) and concentrated fruit and berry juices of black currant and blueberry (31-37%). The content of iodine was determined by titrimetric method, fucoidan, the sum of phenolic compounds, flavonoids, catechins - by spectrophotometric method, vitamin C - titrimetrically, anthocyanins - by pH-differential spectrophotometry. Identification of phenolic compounds was carried out by HPLC. The antiradical properties of beverages were evaluated by the ability to interact with the stable 2.2-diphenyl-1-picrylhydrazyl free radical in vitro spectrophotometrically. . Dry hydrothermal extracts of C. costata and U. pinnatifida brown algae are characterized by a high content of bioactive substances, the main of which are fucoidan and iodine. The fucoidan content in C. costata extract was 1.7 g/100 g, in U. pinnatifida extract - 0.5 g/100 g; the iodine content was 0.0036 and 0.0028 g/100 g, respectively. The content of phenolic compounds was at least 205 mg of tannic acid per 1 g, the main compounds were syringic acid and epicatechin, salicylic and coumaric acids, as well as chlorogenic, caffeic, 2.5-dihydroxybenzoic, ferulic acid and gallates of epigallocatechin and epicatechin. The developed drinks based on dry extracts of C. costata and U. pinnatifida brown algae (21-26%) and concentrated blueberry and blackcurrant juices (31-37%) (the rest is powdered sugar) are a food system enriched with bioactive substances. The content per 1 serving of drinks (10 g per 200 ml) of iodine was high and amounted to 70-75 mcg, phenolic compounds - about 250 mg, vitamin C level was maximum in a drink with black currant (41-44 mg), fucoidan content ranged from 79 to 84 mg. The resulting dry drinks satisfy the daily physiological requirement in iodine by at least 40%, in vitamin C - by at least 30% when consuming 1 serving. The radical binding activity of all the studied beverages was quite high and amounted to 91.1-96.5%. The drink with blueberry juice and C. costata extract showed maximum antiradical properties. . The developed dry soft drinks based on dry extracts of C. costata and U. pinnatifida brown algae and concentrated juices of black currant or blueberry contain a wide range of bioactive compounds. They can be attributed to functional products due to the high degree of satisfaction of the daily physiological requirement of the human body in vitamin C and iodine and are characterized by high antiradical properties.

摘要

如今,需要研发不仅能使水-电解质平衡正常化,还能纠正和优化饮食化学结构的功能性饮料。生产干饮料的基础可以是果汁和浆果汁、包括藻类等在内的草药原料提取物。本研究的目的是基于褐藻(海带和裙带菜)的干提取物以及浓缩果汁和浆果汁开发干饮料,并评估其中生物活性物质的含量以及所得饮料的抗氧化性能。远东地区的海带和裙带菜被用作研究对象,从中获得了干热液提取物,以及基于这些提取物(21%-26%)和黑加仑及蓝莓浓缩果汁(31%-37%)的干饮料。碘含量通过滴定法测定,岩藻聚糖、酚类化合物总和、黄酮类化合物、儿茶素通过分光光度法测定,维生素C通过滴定法测定,花青素通过pH差值分光光度法测定。酚类化合物的鉴定通过高效液相色谱法进行。通过体外分光光度法与稳定的2,2-二苯基-1-苦基肼自由基相互作用的能力来评估饮料的抗自由基性能。海带和裙带菜褐藻的干热液提取物具有高含量的生物活性物质,其中主要是岩藻聚糖和碘。海带提取物中岩藻聚糖含量为1.7 g/100 g,裙带菜提取物中为0.5 g/100 g;碘含量分别为0.0036 g/100 g和0.0028 g/100 g。酚类化合物含量至少为每1 g含205 mg单宁酸,主要化合物有丁香酸和表儿茶素、水杨酸和香豆酸,以及绿原酸、咖啡酸、2,5-二羟基苯甲酸、阿魏酸和表没食子儿茶素及表儿茶素的没食子酸酯。基于海带和裙带菜褐藻干提取物(21%-26%)以及浓缩蓝莓和黑加仑汁(31%-37%)(其余为糖粉)开发的饮料是富含生物活性物质的食品体系。每份饮料(200 ml中10 g)中碘含量很高,达70 - 75 μg,酚类化合物约250 mg,黑加仑汁饮料中维生素C水平最高(41 - 44 mg),岩藻聚糖含量在79至84 mg之间。所得干饮料在饮用1份时至少满足人体每日碘生理需求量的40%,维生素C需求量的至少30%。所有研究饮料的自由基结合活性都相当高,达91.1% - 96.5%。蓝莓汁和海带提取物饮料表现出最大的抗自由基性能。基于海带和裙带菜褐藻干提取物以及黑加仑或蓝莓浓缩汁开发的干软饮料含有多种生物活性化合物。由于它们高度满足人体对维生素C和碘的每日生理需求量,可被归类为功能性产品,并具有高抗自由基性能。

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