大肠杆菌核糖体中蛋白质L7/L12的结构研究。

Studies on the structure of protein L7/L12 from Escherichia coli ribosomes.

作者信息

Gudkov A T, Khechinashvili N N, Bushuev V N

出版信息

Eur J Biochem. 1978 Oct;90(2):313-8. doi: 10.1111/j.1432-1033.1978.tb12606.x.

DOI:10.1111/j.1432-1033.1978.tb12606.x

Abstract

Circular dichroism, infrared and proton magnetic resonance spectroscopy as well as microcalorimetry methods were used to investigate the intact proteins L7/L12 in solution and their different derivatives (L7 with oxidized residues of methionine, fragments 27--120, 1--73 and 74--120)- On the basis of the data obtained the following conclusions have been drawn: (a) there is no beta structure in the protein L7, (B) the N-terminal region of L7 forms a long alpha helix (c) the Phe-30 residue within the N-terminal region of L7 takes part in the dimerization, (d) the C-terminal of L7 is globular and (e) the Phe-54 residue is included in the hydrophobic core of the globular C-terminal region.

摘要

利用圆二色性、红外光谱、质子磁共振光谱以及微量量热法等手段，对溶液中的完整蛋白质L7/L12及其不同衍生物（甲硫氨酸残基氧化的L7、片段27 - 120、1 - 73和74 - 120）进行了研究。基于所获数据得出以下结论：（a）蛋白质L7中不存在β结构；（b）L7的N端区域形成一个长α螺旋；（c）L7的N端区域内的苯丙氨酸-30残基参与二聚化；（d）L7的C端呈球状；（e）苯丙氨酸-54残基包含在球状C端区域的疏水核心中。