State Key Laboratory of Crop Genetics and Germplasm Enhancement, MOA Key Laboratory of Plant Nutrition and Fertilization in Low-Middle Reaches of the Yangtze River, Nanjing Agricultural University, Nanjing 210095, China.
Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
Int J Mol Sci. 2022 Sep 16;23(18):10795. doi: 10.3390/ijms231810795.
The and isoforms play important roles in the uptake and transport of nitrate during rice growth. However, it is unclear which cis-acting element controls the transcription of into these specific isoforms. In this study, we used a yeast one-hybrid assay to obtain the TATA-box binding protein OsTBP2.1, which binds to the TATA-box of , and verified its important role through transient expression and RNA-seq. We found that the TATA-box of mutants and binding protein OsTBP2.1 together increased the transcription ratio of to . The overexpression of promoted nitrogen uptake and increased rice yield compared with the wild-type; however, the T-DNA mutant lines exhibited the opposite trend. Detailed analyses demonstrated that the TATA-box was the key cis-regulatory element for to be transcribed into and . Additionally, this key cis-regulatory element, together with the binding protein OsTBP2.1, promoted the development of rice and increased grain yield.
和 同工型在水稻生长过程中硝酸盐的摄取和转运中发挥重要作用。然而，目前尚不清楚哪个顺式作用元件控制 转录为这些特定的同工型。在这项研究中，我们使用酵母单杂交测定法获得了 TATA 结合蛋白 OsTBP2.1，它与 的 TATA 盒结合，并通过瞬时表达和 RNA-seq 验证了其重要作用。我们发现，突变体和结合蛋白 OsTBP2.1 的 TATA 盒一起增加了 的转录比率。与野生型相比， 的过表达促进了氮的吸收并增加了水稻的产量；然而， T-DNA 突变体系则表现出相反的趋势。详细分析表明，TATA 盒是 将 转录为 和 的关键顺式调控元件。此外，这个关键的顺式调控元件与结合蛋白 OsTBP2.1 一起促进了水稻的发育并增加了籽粒产量。
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