Institute of Entomology, Guizhou University, Guizhou Provincial Key Laboratory for Agricultural Pest Management in the Mountainous Region, Guiyang 550025, China.
Int J Mol Sci. 2022 Sep 17;23(18):10886. doi: 10.3390/ijms231810886.
The western flower thrips, Pergande, is an invasive pest that damages agricultural and horticultural crops. The induction of plant defenses and RNA interference (RNAi) technology are potent pest control strategies. This study investigated whether the anti-adaptive ability of . to jasmonic acid (JA)- and methyl jasmonate (MeJA)-induced defenses in kidney bean plants was attenuated after glutathione S-transferase (GST) gene knockdown. The expression of four GSTs in thrips fed JA- and MeJA-induced leaves was analyzed, and and were upregulated. Exogenous JA- and MeJA-induced defenses led to increases in defensive secondary metabolites (tannins, alkaloids, total phenols, flavonoids, and lignin) in leaves. Metabolome analysis indicated that the JA-induced treatment of leaves led to significant upregulation of defensive metabolites. The activity of GSTs increased in second-instar thrips larvae fed JA- and MeJA-induced leaves. Co-silencing with RNAi simultaneously knocked down and transcripts and GST activity, and the area damaged by second-instar larvae feeding on JA- and MeJA-induced leaves decreased by 62.22% and 55.24%, respectively. The pupation rate of second-instar larvae also decreased by 39.68% and 39.89%, respectively. Thus, RNAi downregulation of and reduced the anti-adaptive ability of . to JA- or MeJA-induced defenses in kidney bean plants.
西方花蓟马（Pergande）是一种入侵性害虫，会损害农业和园艺作物。植物防御的诱导和 RNA 干扰（RNAi）技术是有效的害虫控制策略。本研究调查了谷胱甘肽 S-转移酶（GST）基因敲低后，是否会减弱 对绿豆植株中茉莉酸（JA）和甲基茉莉酸（MeJA）诱导防御的抗适应性。分析了 JA 和 MeJA 诱导叶片中四种 GSTs 的表达情况， 和 上调。外源 JA 和 MeJA 诱导的防御导致叶片中防御性次生代谢物（单宁、生物碱、总酚、类黄酮和木质素）增加。代谢组学分析表明，JA 诱导处理叶片导致防御代谢物显著上调。JA 和 MeJA 诱导叶片中 GSTs 的活性在二龄幼虫中增加。RNAi 共沉默同时敲低 和 转录物和 GST 活性，二龄幼虫取食 JA 和 MeJA 诱导叶片的损伤面积分别减少 62.22%和 55.24%。二龄幼虫的化蛹率也分别下降了 39.68%和 39.89%。因此，RNAi 下调 和 降低了 对绿豆植株中 JA 或 MeJA 诱导防御的抗适应性。
