Li Ye, Zhang Ruiqiong, Sun Lili, Cao Chuanwang
Key Laboratory of Sustainable Forest Ecosystem Management-Ministry of Education, Northeast Forestry University, Harbin, 150040, China.
Transgenic Res. 2025 Jan 9;34(1):10. doi: 10.1007/s11248-024-00426-5.
Lignin is a crucial defense phytochemical against phytophagous insects. Cinnamoyl-CoA reductase (CCR) is a key enzyme in lignin biosynthesis. In this study, transgenic Populus davidiana × P. bolleana overexpressing the PdbCCR gene were generated via Agrobacterium-mediated transformation. Successful integration of PdbCCR into the poplar genome was confirmed by PCR amplification and quantitative reverse transcription PCR (qRT-PCR). The lignin content in the transgenic poplar leaves was significantly higher than that in the wild poplar, and after L. dispar larvae fed on the transgenic poplar, the CCR activity was clearly induced. The L. dispar larvae grew slowly after feeding on transgenic poplar and the laccase, cellulase and three detoxifying enzymes were induced compared with larvae after feeding on wild-type poplar. The bioassay further revealed that transgenic poplar plants overexpressing PdbCCR showed a high level of resistance to L. dispar larvae. These results confirmed that PdbCCR is a candidate gene for breeding insect resistant poplar.
木质素是一种对抗植食性昆虫的关键防御性植物化学物质。肉桂酰辅酶A还原酶（CCR）是木质素生物合成中的关键酶。在本研究中，通过农杆菌介导的转化产生了过表达PdbCCR基因的转基因山杨×小叶杨。通过PCR扩增和定量逆转录PCR（qRT-PCR）证实了PdbCCR成功整合到杨树基因组中。转基因杨树叶片中的木质素含量显著高于野生杨树，在舞毒蛾幼虫取食转基因杨树后，CCR活性明显被诱导。与取食野生型杨树的幼虫相比，舞毒蛾幼虫取食转基因杨树后生长缓慢，漆酶、纤维素酶和三种解毒酶被诱导。生物测定进一步表明，过表达PdbCCR的转基因杨树植株对舞毒蛾幼虫具有高度抗性。这些结果证实PdbCCR是培育抗虫杨树的候选基因。
