Yang Wenshuo, Xu Liang, Wang Zhao, Li Kai, Hu Ruizhu, Su Junfeng, Zhang Lingfei
School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China; Shaanxi Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China.
School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China; Shaanxi Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China.
Bioresour Technol. 2022 Nov;363:127996. doi: 10.1016/j.biortech.2022.127996. Epub 2022 Sep 20.
Pseudomonas sp. Y1, a strain with superior synchronous removal ability of ammonia nitrogen (NH-N), phosphate (PO-P), and calcium (Ca) was isolated, with the removal efficiencies of 92.04, 99.98, and 83.40 %, respectively. Meanwhile, the chemical oxygen demand (COD) was degraded by 90.33 %. Through kinetic analysis, the optimal cultivated conditions for heterotrophic nitrification-aerobic denitrification (HNAD) and biomineralization were determined. The growth curves experimental results of different nitrogen sources indicated that strain Y1 could remove NH-N through HNAD. The results of excitation-emission matrix (EEM) proved that the appearance of extracellular polymeric substances (EPS) promoted the precipitation of phosphate minerals. Finally, the characterization results of the bioprecipitates showed that the HNAD process produced the alkalinity required for microbial induced calcium precipitation (MICP), resulting in the removal of PO via adsorption and co-precipitation. This study provides a theoretical basis for the application of microorganisms to achieve synchronous nutrient removal and phosphorus recovery in wastewater.
假单胞菌属菌株Y1是一种对氨氮（NH-N）、磷酸盐（PO-P）和钙（Ca）具有卓越同步去除能力的菌株，其去除效率分别为92.04%、99.98%和83.40%。同时，化学需氧量（COD）降解了90.33%。通过动力学分析，确定了异养硝化-好氧反硝化（HNAD）和生物矿化的最佳培养条件。不同氮源的生长曲线实验结果表明，菌株Y1可通过HNAD去除NH-N。激发-发射矩阵（EEM）结果证明，胞外聚合物（EPS）的出现促进了磷酸盐矿物的沉淀。最后，生物沉淀物的表征结果表明，HNAD过程产生了微生物诱导钙沉淀（MICP）所需的碱度，从而通过吸附和共沉淀去除PO。本研究为微生物在废水中实现同步营养物去除和磷回收的应用提供了理论依据。
