放射性自显影中 GTPγS 结合的分析。
Assay of GTPγS Binding in Autoradiography.
机构信息
Department of Biotechnology and Life Sciences, University of Insubria, Busto Arsizio, (VA), Italy.
Fondazione Zardi-Gori, Milano, Italy.
出版信息
Methods Mol Biol. 2023;2576:181-188. doi: 10.1007/978-1-0716-2728-0_14.
PMID:36152186
Abstract
Autoradiography of radiolabeled GTPγS ([S]GTPγS) binding is a relevant technique to study the function of G protein-coupled receptors (GPCRs) ex vivo. Here, we describe the protocol for such a method, suitable for investigating CB receptor functionality in tissue slices from rodent brains.
摘要
放射性标记的 GTPγS([S]GTPγS)结合的放射自显影是一种研究 G 蛋白偶联受体 (GPCR) 体外功能的相关技术。本文描述了一种适用于研究啮齿动物脑切片中 CB 受体功能的方法。
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