European Center for Brain Research/Santa Lucia Foundation IRCCS, Rome, Italy.
Department of Experimental Medicine, Tor Vergata University of Rome, Rome, Italy.
Methods Mol Biol. 2023;2576:299-305. doi: 10.1007/978-1-0716-2728-0_25.
The α,β-hydrolase fold-containing protein 2 (ABHD2) is a serine hydrolase, responsible for the cleavage of endogenous 2-arachidonoylglycerol (2-AG). ABHD2 is activated by progesterone, thus, it is considered a nonnuclear receptor of this steroid hormone that terminates its biological effects. The products of ABHD2-catalyzed cleavage by the natural substrate 2-AG are glycerol and arachidonic acid; here, instead of 2-AG, the radioactive substrate 2-oleoyl-[H]glycerol has been used as already done in various acylglycerol lipase activity assays. The amount of [H]glycerol released allows to measure ABHD2 enzymatic activity.
α,β-水解酶折叠蛋白 2 (ABHD2) 是一种丝氨酸水解酶，负责分解内源性 2-花生四烯酸甘油 (2-AG)。ABHD2 被孕激素激活，因此，它被认为是这种类固醇激素的非核受体，终止其生物效应。ABHD2 催化天然底物 2-AG 水解的产物是甘油和花生四烯酸；在这里，已经在各种甘油酯脂肪酶活性测定中完成的那样，使用放射性底物 2-油酰-[H]甘油代替 2-AG。释放的 [H]甘油的量允许测量 ABHD2 酶活性。
