脂氧合酶对大麻素的氧化作用。
Oxygenation of Anandamide by Lipoxygenases.
机构信息
Cellular Protein Chemistry, Faculty of Science, Utrecht University, Utrecht, The Netherlands.
Department Molecular Physiology, Leiden Institute of Chemistry, Leiden University, Leiden, The Netherlands.
出版信息
Methods Mol Biol. 2023;2576:307-316. doi: 10.1007/978-1-0716-2728-0_26.
The endocannabinoids anandamide and 2-arachidonoylglycerol are not only metabolized by serine hydrolases, such as fatty acid amide hydrolase, monoacylglycerol lipase, and α,β-hydrolases 6 and 12, but they also serve as substrates for cyclooxygenases, cytochrome P450s, and lipoxygenases. These enzymes oxygenate the 1Z,4Z-pentadiene system of the arachidonic acid backbone of endocannabinoids, thereby giving rise to an entirely new array of bioactive lipids. Hereby, a protocol is provided for the enzymatic synthesis, purification, and characterization of various oxygenated metabolites of anandamide generated by lipoxygenases, which enables the biological study and detection of these metabolites.
内源性大麻素大麻酰胺和 2-花生四烯酰甘油不仅可被丝氨酸水解酶（如脂肪酸酰胺水解酶、单酰甘油脂肪酶和 α,β-水解酶 6 和 12）代谢，还可作为环加氧酶、细胞色素 P450 和脂加氧酶的底物。这些酶氧化内源性大麻素花生四烯酸主链的 1Z,4Z-戊二烯体系，从而产生全新的生物活性脂质。本协议提供了脂加氧酶生成的大麻酰胺各种氧化代谢物的酶促合成、纯化和表征方法，使这些代谢物的生物学研究和检测成为可能。
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