尼罗红和 BODIPY 染色法检测小鼠卵母细胞和胚胎中的脂滴。

Nile Red and BODIPY Staining of Lipid Droplets in Mouse Oocytes and Embryos.

机构信息

Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland.

出版信息

Methods Mol Biol. 2023;2566:205-212. doi: 10.1007/978-1-0716-2675-7_16.

Abstract

Lipid droplets (LDs) are intracellular structures composed of hydrophobic lipids. Their amount in oocytes and embryos varies among the mammalian species and even among different strains of the same species. Here we describe a method to stain LDs, which can be applied to previously fixed mouse oocytes and embryos. This method is based on fluorescent dyes, Nile red and BODIPY, which allow visualization and quantification of LDs using conventional and confocal fluorescence microscopy.

摘要

脂滴（LDs）是由疏水脂质组成的细胞内结构。它们在卵母细胞和胚胎中的数量在哺乳动物物种之间甚至在同一物种的不同品系之间都有所不同。在这里，我们描述了一种可以对先前固定的小鼠卵母细胞和胚胎进行染色的 LD 染色方法。该方法基于荧光染料尼罗红和 BODIPY，可使用常规和共聚焦荧光显微镜对 LD 进行可视化和定量。