胶原诱导关节炎小鼠模型的标准化方案及结果评估。

Standardized protocol and outcome measurements for the collagen antibody-induced arthritis mouse model.

机构信息

Charité - Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Center for Musculoskeletal Surgery, 13353 Berlin, Germany; Berlin Institute of Health at Charité - Universitätsmedizin Berlin, Julius Wolff Institute, 13353 Berlin, Germany; Berlin Institute of Health at Charité - Universitätsmedizin Berlin, BIH Biomedical Innovation Academy, BIH Charité Clinician Scientist Program, 10178 Berlin, Germany.

出版信息

STAR Protoc. 2022 Dec 16;3(4):101718. doi: 10.1016/j.xpro.2022.101718. Epub 2022 Sep 22.

DOI:10.1016/j.xpro.2022.101718

PMID:36152302

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9519592/

Abstract

The murine collagen antibody-induced arthritis (CAIA) model resembles various features of human rheumatoid arthritis and is based on the intraperitoneal or intravenous injection of autoantibodies against type II collagen. Here, we present a standardized protocol for the intraperitoneal injection of arthritis-inducing autoantibodies in mice, followed by a description of daily arthritis assessments. We then detail the steps to harvest joint and bone tissues for histological, radiological, and molecular analyses. We highlight animal welfare and 3 considerations for experimental arthritis studies. For complete details on the use and execution of this protocol, please refer to Maleitzke et al. (2021, 2022).

摘要

鼠胶原抗体诱导关节炎（CAIA）模型类似于人类类风湿关节炎的各种特征，基于针对 II 型胶原的自身抗体的腹腔内或静脉内注射。在此，我们提供了一种在小鼠中进行关节炎诱导自身抗体腹腔内注射的标准化方案，随后描述了每日关节炎评估。然后，我们详细介绍了用于组织学、影像学和分子分析的关节和骨骼组织采集步骤。我们强调了动物福利和实验性关节炎研究的 3 个注意事项。有关此方案的使用和执行的完整详细信息，请参阅 Maleitzke 等人（2021 年，2022 年）。