Stenstrand K, Larsen J K, Wulf H C, Lange Wantzin G
Photodermatol. 1987 Feb;4(1):36-42.
Isolated human peripheral blood lymphocytes were exposed to 254 nm UV light and stimulated with PHA. Chromosome analysis revealed significant increases in sister chromatid exchanges (SCE's) and micronuclei, and also in gross chromosomal aberrations, but not in breaks and gaps on the chromatid level. By means of flow cytometry it was shown that UV exposure perturbed the cell cycle in that the S and G2/M fraction decreased when UV was given before stimulation with PHA. Labelling the cells with fluorescein-conjugated antibodies did not reveal any changes in the relative frequencies of lymphocyte subsets after exposure, but an overall diminished ability to bind antibody was observed.
将分离出的人外周血淋巴细胞暴露于254纳米紫外线，并使用PHA进行刺激。染色体分析显示姐妹染色单体交换（SCE）、微核以及染色体严重畸变显著增加，但染色单体水平的断裂和裂隙未增加。通过流式细胞术表明，紫外线照射扰乱了细胞周期，即在用PHA刺激前给予紫外线时，S期和G2/M期细胞比例降低。用荧光素偶联抗体标记细胞后，未发现暴露后淋巴细胞亚群的相对频率有任何变化，但观察到总体抗体结合能力下降。
