Scheibner A, Hollis D E, Murray E, McCarthy W H, Milton G W
Photodermatol. 1987 Feb;4(1):5-13.
The effects of 3 daily, 7-min exposures to artificial ultraviolet light (UVL), designed to simulate natural sunlight, on epidermal Langerhans cells (LC) and melanocytes were studied in 17 healthy Australian volunteers of differing skin pigmentation. Six were of Celtic, 6 of Asian and 5 of Aboriginal descent. LC were visualized using the immunofluorescence method for HLA-DR and T6 antigens, and the histochemical method for ATPase. UVL induced a transient reduction in the LC population density and an increase in the number of melanocytes in all subject groups. The reduction in number of immunocompetent LC or the disruption of their surface markers was greatest in the Celtic subjects, who had the fairest skin, and least in the Aboriginal and Asian subjects, who had the darkest skin. However, neither the inherently dark skin pigmentation nor the UVL-induced increase in pigmentation were sufficient to prevent the depletion of immunocompetent epidermal LC. Non-dendritic, rounded cells and very large dendritic, non-LC, which were present in the epidermis of some subjects, were stimulated to increase in number by exposure to UVL. The identity and function of these cells is uncertain and they require further investigation.
在17名不同皮肤色素沉着的健康澳大利亚志愿者中，研究了每日3次、每次7分钟暴露于旨在模拟自然阳光的人工紫外线（UVL）对表皮朗格汉斯细胞（LC）和黑素细胞的影响。其中6人是凯尔特血统，6人是亚洲血统，5人是原住民血统。使用针对HLA - DR和T6抗原的免疫荧光法以及ATP酶的组织化学法对LC进行可视化。UVL在所有受试组中均导致LC群体密度短暂降低，黑素细胞数量增加。具有最白皙皮肤的凯尔特受试者中，免疫活性LC数量的减少或其表面标志物的破坏最为明显，而皮肤最黑的原住民和亚洲受试者中则最少。然而，无论是天生的深色皮肤色素沉着还是UVL诱导的色素沉着增加，都不足以防止免疫活性表皮LC的消耗。一些受试者表皮中存在的非树突状圆形细胞以及非常大的树突状非LC细胞，通过暴露于UVL刺激其数量增加。这些细胞的身份和功能尚不确定，需要进一步研究。
