甲基乙酰磷酸对血红蛋白S的影响：一种针对二磷酸甘油酸（DPG）结合位点的新型乙酰化剂。
Effects of methyl acetyl phosphate on hemoglobin S: a novel acetylating agent directed towards the DPG binding site.
作者信息
Ueno H, Benjamin L J, Manning J M
出版信息
Prog Clin Biol Res. 1987;240:105-10.
PMID:3615481
Abstract
Methyl acetyl phosphate (MAP) is an acetylating agent which prevents the polymerization of sickle cell hemoglobin (HbS). MAP can penetrate sickle erythrocytes and can acetylate intracellular HbS. Tryptic peptide mapping of modified HbS on reversed phase HPLC and amino acid analysis of isolated peptides revealed the acetylation sites to be Val-1 (beta), Lys-82 (beta), and Lys-144 (beta). MAP, therefore, is specifically directed towards the 2,3-DPG binding site of Hb.
摘要
甲基乙酰磷酸（MAP）是一种乙酰化剂，可阻止镰状细胞血红蛋白（HbS）聚合。MAP能够穿透镰状红细胞并使细胞内的HbS乙酰化。经反相高效液相色谱法对修饰后的HbS进行胰蛋白酶肽图谱分析以及对分离出的肽段进行氨基酸分析，结果显示乙酰化位点为缬氨酸-1（β链）、赖氨酸-82（β链）和赖氨酸-144（β链）。因此，MAP特异性作用于Hb的2,3-二磷酸甘油酸（2,3-DPG）结合位点。
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