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姜黄素和辣椒素调节细胞凋亡，缓解艰难梭菌诱导的体外肠道炎症。

Curcumin and capsaicin regulate apoptosis and alleviate intestinal inflammation induced by Clostridioides difficile in vitro.

机构信息

Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

出版信息

Ann Clin Microbiol Antimicrob. 2022 Sep 26;21(1):41. doi: 10.1186/s12941-022-00533-3.

DOI:10.1186/s12941-022-00533-3
PMID:36155114
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9511736/
Abstract

BACKGROUND: The dramatic upsurge of Clostridioides difficile infection (CDI) by hypervirulent isolates along with the paucity of effective conventional treatment call for the development of new alternative medicines against CDI. The inhibitory effects of curcumin (CCM) and capsaicin (CAP) were investigated on the activity of toxigenic cell-free supernatants (Tox-S) of C. difficile RT 001, RT 126 and RT 084, and culture-filtrate of C. difficile ATCC 700057. METHODS: Cell viability of HT-29 cells exposed to varying concentrations of CCM, CAP, C. difficile Tox-S and culture-filtrate was assessed by MTT assay. Anti-inflammatory and anti-apoptotic effects of CCM and CAP were examined by treatment of HT-29 cells with C. difficile Tox-S and culture-filtrate. Expression of BCL-2, SMAD3, NF-κB, TGF-β and TNF-α genes in stimulated HT-29 cells was measured using RT-qPCR. RESULTS: C. difficile Tox-S significantly (P < 0.05) reduced the cell viability of HT-29 cells in comparison with untreated cells. Both CAP and CCM significantly (P < 0.05) downregulated the gene expression level of BCL-2, SMAD3, NF-κB and TNF-α in Tox-S treated HT-29 cells. Moreover, the gene expression of TGF-β decreased in Tox-S stimulated HT-29 cells by both CAP and CCM, although these reductions were not significantly different (P > 0.05). CONCLUSION: The results of the present study highlighted that CCM and CAP can modulate the inflammatory response and apoptotic effects induced by Tox-S from different clinical C. difficile strains in vitro. Further studies are required to accurately explore the anti-toxin activity of natural components, and their probable adverse risks in clinical practice.

摘要

背景：随着产毒能力更强的艰难梭菌（Clostridioides difficile）分离株的急剧增加以及缺乏有效的传统治疗方法，需要开发新的替代药物来治疗艰难梭菌感染（CDI）。本研究旨在研究姜黄素（CCM）和辣椒素（CAP）对产毒无细胞上清液（Tox-S）和艰难梭菌 ATCC 700057 培养液的抑制作用。

方法：通过 MTT 法评估 HT-29 细胞暴露于不同浓度的 CCM、CAP、艰难梭菌 Tox-S 和培养液后的细胞活力。通过用艰难梭菌 Tox-S 和培养液处理 HT-29 细胞，检测 CCM 和 CAP 的抗炎和抗凋亡作用。使用 RT-qPCR 测量刺激 HT-29 细胞中 BCL-2、SMAD3、NF-κB、TGF-β 和 TNF-α 基因的表达。

结果：与未经处理的细胞相比，艰难梭菌 Tox-S 显著（P < 0.05）降低了 HT-29 细胞的活力。CAP 和 CCM 均显著（P < 0.05）下调了 Tox-S 处理的 HT-29 细胞中 BCL-2、SMAD3、NF-κB 和 TNF-α 基因的表达水平。此外，CAP 和 CCM 均使 Tox-S 刺激的 HT-29 细胞中 TGF-β 的基因表达降低，尽管这些降低无统计学差异（P > 0.05）。

结论：本研究结果表明，CCM 和 CAP 可以调节体外不同临床艰难梭菌菌株的 Tox-S 诱导的炎症反应和细胞凋亡作用。需要进一步研究以准确探索天然成分的抗毒素活性及其在临床实践中的可能不良反应风险。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad55/9511736/6bb678b692d4/12941_2022_533_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad55/9511736/0c766fe8dec5/12941_2022_533_Fig1_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad55/9511736/2a41c894a0f8/12941_2022_533_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad55/9511736/6bb678b692d4/12941_2022_533_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad55/9511736/0c766fe8dec5/12941_2022_533_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad55/9511736/66923bb6528c/12941_2022_533_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad55/9511736/fc132d0e3380/12941_2022_533_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad55/9511736/3be462fab7be/12941_2022_533_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad55/9511736/47a3b2cc0ffe/12941_2022_533_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad55/9511736/1197f4ae4c04/12941_2022_533_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad55/9511736/2a41c894a0f8/12941_2022_533_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad55/9511736/6bb678b692d4/12941_2022_533_Fig8_HTML.jpg

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[5]
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[7]
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本文引用的文献

[1]
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Antibiotics (Basel). 2022-2-28

[2]
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Front Microbiol. 2021-12-21

[3]
Capsaicin attenuates TGFβ2-induced epithelial-mesenchymal-transition in lens epithelial cells in vivo and in vitro.

Exp Eye Res. 2021-12

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Emerg Microbes Infect. 2020-12

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